Weissella, a novel lactic acid bacteria isolated from wild Sumatran orangutans (Pongo abelii)

Vet World Vol.12 July-2019 Article-20

Research Article

Veterinary World, 12(7): 1060-1065

https://doi.org/10.14202/vetworld.2019.1060-1065

Safika Safika¹, Wardinal Wardinal², Yulia Sari Ismail³, Khairun Nisa², and Wenny Novita Sari⁴

1. Department of Veterinary Infection Diseases and Veterinary Public Health, Faculty of Veterinary Medicine, IPB University, Jalan Agatis, IPB Dermaga, Bogor 16680, Indonesia.
2. Department of Biology, Education and Teaching Faculty, Ar-Raniry State Islamic University Banda Aceh 23111, Indonesia.
3. Department of Biology, Faculty of Mathematics and Sciences, Syiah Kuala University, Banda Aceh 23111, Indonesia.
4. Department of Mathematics and Applied Sciences, Syiah Kuala University, Darussalam, Banda Aceh 23111, Indonesia.

Background and Aim: This study aimed to isolate and identify lactic acid bacteria (LAB) in wild Sumatran orangutans to provide more information about LAB diversity derived from Sumatran orangutan feces.

Materials and Methods: Fecal sampling from three female orangutans, around 35 years old, was carried out in the wild forest areas at the research station of Suaq Belimbing Gunung Leuser National Park located in the South Aceh district. Orangutan fecal samples were taken in the morning when the orangutans first defecated. The orangutans were above the tree, which is approximately 12-15 m from the ground where feces were found.

Results: Fermentation testing using the API 50 CHL Kit showed that OUL4 isolates were identified as Lactococcus lactis ssp. lactis with an identity value of 73.5%. Homology analysis demonstrated that the OUL4 isolates have 93% similarity to Weissella cibaria, and phylogenetic trees constructed using Mega 7.0 also showed that OUL4 isolates are related to W. cibaria.

Conclusion: These results show that there is a difference in identification between biochemical testing with API kits and molecular analyses on LAB isolates from wild Sumatran orangutans. Based on 16S rRNA gene homology, the OUL4 LAB isolates from wild Sumatran orangutans have 93% homology to W. cibaria. Keywords: 16S rRNA, API 50 CHL, lactic acid bacteria, orangutan, Pongo abelii, Weissella.

Keywords: 16S rRNA, API 50 CHL, lactic acid bacteria, orangutan, Pongo abelii, Weissella.

How to cite this article: Safika S, Wardinal W, Ismail YS, Nisa K, Sari WN (2019) Weissella, a novel lactic acid bacteria isolated from wild Sumatran orangutans (Pongo abelii), Veterinary World, 12(7): 1060-1065.

Received: 07-01-2019 Accepted: 10-05-2019 Published online: 18-07-2019

Corresponding author: Safika Safika E-mail: fikakhan@yahoo.com

DOI: 10.14202/vetworld.2019.1060-1065

Copyright: Safika, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.