Vet World Vol.12 March-2019 Article-14
Research Article
Veterinary World, 12(3): 440-448
https://doi.org/10.14202/vetworld.2019.440-448
Nanoparticles of zinc oxide defeat chlorpyrifos-induced immunotoxic effects and histopathological alterations
2. Department of Toxicology, Forensic Medicine and Veterinary Regulations, Faculty of Veterinary Medicine, Cairo University, Egypt.
3. Department of Pathology, Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt.
Background and Aim: Chlorpyrifos (CPF) is a widely used organophosphate insecticide. Nanoparticles of zinc oxide (ZnO NPs) physically showed effective adsorbing property for some insecticides. The study was conducted to estimate the potential effect of ZnO NPs against CPF toxicity.
Materials and Methods: Four groups of male rats were used; control group and three groups received drinking water contained 75 mg/L CPF, combined 75 mg/L CPF and 200 mg/L ZnO NPs, and 200 mg/L ZnO NPs, respectively.
Results: CPF significantly decreased macrophage activity, serum lysozyme activity, and levels of interleukin-2 (IL-2) and IL-6; increased the percentage of DNA degeneration on comet assay of lymphocytes and significantly elevated hepatic and splenic malondialdehyde contents; and decreased their glutathione contents. The liver and spleen showed marked histological alterations after exposure to CPF with decreased expression of acetylcholinesterase. The coadministration of ZnO NPs ameliorated most of the undesirable effects of CPF, through elevation of macrophage and serum lysozyme activities, increased the levels of IL-2 and IL-6, corrected the oxidative stress markers, and alleviated most of the adverse effect exerted by CPF in liver and spleen tissues.
Conclusion: The addition of ZnO NPs to CPF-contaminated drinking water may be useful as a powerful antioxidant agent against toxic damage induced by CPF particularly in individuals who are on daily occupational exposure to low doses of CPF. Keywords: acetylcholinesterase, chlorpyrifos, immune system, pathology, zinc oxide nanoparticles.
Keywords: acetylcholinesterase, chlorpyrifos, immune system, pathology, zinc oxide nanoparticles.
How to cite this article: Essa SS, El-Saied EM, El-Tawil OS, Gamal IM, Abd El-Rahman SS (2019) Nanoparticles of zinc oxide defeat chlorpyrifos-induced immunotoxic effects and histopathological alterations, Veterinary World, 12(3): 440-448.
Received: 02-12-2018 Accepted: 28-01-2019 Published online: 22-03-2019
Corresponding author: Sahar S. Abd El-Rahman E-mail: saharsamirmah@cu.edu.eg
DOI: 10.14202/vetworld.2019.440-448
Copyright: Essa, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.