Vet World Vol.12 November-2019 Article-14
Research Article
Veterinary World, 12(11): 1784-1789
https://doi.org/10.14202/vetworld.2019.1784-1789
Addition of L-arginine in skim milk extender maintains goat spermatozoa quality in chilled temperature for five days
2. Department of Veterinary Pathology, Faculty of Veterinary Medicine, Universitas Airlangga, Kampus C Unair, Jl. Mulyorejo, Surabaya - 60115, Surabaya, Indonesia.
Background and Aim: The purpose of this study was to determine the benefits of L-arginine addition in skim milk extender to maintain the quality of goat spermatozoa in chilled storage.
Materials and Methods: A total of 18 ejaculates from three healthy goats with weight and age of 45 kg and 4-5 years, respectively, were divided into three groups. The control group contained goat semen diluted in a skim milk extender without L-arginine; Treatment I and Treatment II contained goat semen diluted in a skim milk extender with added L-arginine 4 and 6 mM, respectively. These three groups were chilled at 5°C and evaluated daily for 5 days. Observed variables were viability, motility, intact plasma membrane (IPM), malondialdehyde (MDA) level, necrosis, and apoptosis of spermatozoa.
Results: The addition of L-arginine 4 mM was the best treatment in maintaining viability, motility, and IPM and a decreased MDA level, percentage of necrosis, and apoptosis of goat spermatozoa. An ejaculate in this extender can be divided into 37 doses for intracervical insemination in <1 ml volume with 125 million motile spermatozoa.
Conclusion: Goat semen retained its quality when kept for 5 days in chilled storage by adding L-arginine in skim milk extender. Keywords: apoptosis, goat spermatozoa, L-arginine, malondialdehyde level, necrosis.
Keywords: apoptosis, goat spermatozoa, L-arginine, malondialdehyde level, necrosis.
How to cite this article: Susilowati S, Triana IN, Wurlina W, Arimbi A, Srianto P, Mustofa I (2019) Addition of L-arginine in skim milk extender maintains goat spermatozoa quality in chilled temperature for five days, Veterinary World, 12(11):1784-1789.
Received: 29-07-2019 Accepted: 11-10-2019 Published online: 15-11-2019
Corresponding author: Imam Mustofa E-mail: imam.mustofa@fkh.unair.ac.id
DOI: 10.14202/vetworld.2019.1784-1789
Copyright: Susilowati, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.