Vet World   Vol.12   November-2019  Article-23

Relationship between certain serum biochemical values and serostatus against Anaplasma marginale in dairy cows

Background and Aim: This study was conducted to evaluate the possible association between values of certain serum biochemical parameters and seropositivity against Anaplasma marginale in dairy cows.

Materials and Methods: Serum samples from 60 seropositive and 40 seronegative cows were used to determine the values of beta-hydroxybutyrate (BHB), glucose, creatinine, blood urea nitrogen, total protein, albumin, alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine transaminase (ALT), lactate dehydrogenase (LDH), and gamma-glutamyl transferase (GGT) using commercially available kits and reagents. The serostatus of cows against A. marginale was determined using a commercially available cellular enzyme-linked immunosorbent assay kit according to the manufacturer's recommendations. Significant differences in serum biochemical values between seropositive and seronegative groups were evaluated using independent Student's t-test. Possible associations between the serostatus of the cows and different biochemical parameters were evaluated using univariate followed by multivariate logistic regression analyses.

Results: There was a statistically significant increase (p≤0.05) in values of total protein, BHB, LDH, and AST in seropositive cows compared to seronegative cows while a non-significant increase in values of ALP, ALT, and GGT was detected in seropositive cows. A strong correlation (R=0.69) between serum levels of BHB, LDH, and AST and seropositivity against A. marginale was detected.

Conclusion: There is evidence of a possible association between A. marginale infection and liver damage/hepatic fatty degeneration in dairy cows. Further studies, however, are required to elucidate the exact pathophysiological mechanisms of this relationship. Keywords: blood parasites, dairy cows, hemolysis, metabolic diseases.

Keywords: blood parasites, dairy cows, hemolysis, metabolic diseases.

How to cite this article: Alekish MO, Ismail ZB (2019) Relationship between certain serum biochemical values and serostatus against Anaplasma marginale in dairy cows, Veterinary World, 12(11): 1858-1861.

Received: 10-07-2019  Accepted: 17-10-2019     Published online: 26-11-2019

Corresponding author: Myassar O. Alekish   E-mail: moalekish@just.edu.jo

DOI: 10.14202/vetworld.2019.1858-1861

Copyright: Alekish and Ismail, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.