Vet World Vol.12 November-2019 Article-3
Research Article
Veterinary World, 12(11): 1698-1706
https://doi.org/10.14202/vetworld.2019.1698-1706
Comparing cytotoxicity of propoxur and Nepeta crispa (Lamiales: Lamiaceae) essential oil against invertebrate (Sf9) and vertebrate (L929) cell lines
2. Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
3. Department of Anatomy, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Background and Aim: Attempts to use the plant products are to be an appropriate option due to substantial concerns about human health and environmental problems of using synthetic pesticides. Therefore, the cytotoxicity of Nepeta crispa essential oil was compared with propoxur against invertebrate (Sf9) and vertebrate (L929) cell lines.
Materials and Methods: The cell lines of Sf9 and L929 which were derived from the ovary glands of fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae) and mouse fibroblast cells, respectively, were obtained from the National Cell Bank of Pasteur Institute of Iran. About a number of 2 × 103 cells were placed into the wells of 96-well plate experiments. Then, appropriate concentrations of essential oil of N. crispa plant and propoxur added to the wells. The cells were allowed to grow for 3-5 days and estimated the numbers of cells. The cells of control experiment wells contained only cells with dimethyl sulfoxide. All control and treatment experiments repeated at least four replicates.
Results: Propoxur had negative effects on the viability of both invertebrate (Sf9) and vertebrate (L929) cell lines. The cytotoxicity of propoxur against invertebrate (Sf9) and vertebrate (L929) cell lines was gradually increased in accordance with propoxur concentrations. The cytotoxicity of N. crispa essential oil against vertebrate (L929) cell line was gradually decreased in accordance with plant concentrations, while the cytotoxicity of N. crispa essential oil against invertebrate (Sf9) cell line was strongly increased in accordance with plant concentrations.
Conclusion: Plant essential oil not only had no negative effects but also had boosting effects on vertebrate cell viability. Essential oil of N. crispa plant had negative effects on invertebrate cell viability with the differences that the products derived from plants possessing of biodegradable and environmentally friendly derivatives, hydrolyzing rapidly in nature, and nearly having no destructive effects on environment, humans, or the mammals. Keywords: biodegradable derivative, invertebrate cell line, plant essential oil, plant natural products, vertebrate cell line.
Keywords: biodegradable derivative, invertebrate cell line, plant essential oil, plant natural products, vertebrate cell line.
How to cite this article: Zahirnia A, Boroomand M, Nasirian H, Salehzadeh A, Soleimani-Asl S (2019) Comparing cytotoxicity of propoxur and Nepeta crispa (Lamiales: Lamiaceae) essential oil against invertebrate (Sf9) and vertebrate (L929) cell lines, Veterinary World, 12(11): 1698-1706.
Received: 08-06-2019 Accepted: 30-09-2019 Published online: 04-11-2019
Corresponding author: Aref Salehzadeh E-mail: salehzadeh@umsha.ac.ir
DOI: 10.14202/vetworld.2019.1698-1706
Copyright: Zahirnia, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.