Vet World Vol.12 November-2019 Article-8
Research Article
Veterinary World, 12(11): 1747-1754
https://doi.org/10.14202/vetworld.2019.1747-1754
Multidose intramuscular allogeneic adipose stem cells decrease the severity of canine atopic dermatitis: A pilot study
2. Departamento de Bioquímica y Biología Molecular, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, Spain.
3. Department of Cytometry, Institute of Cell Therapy. CRIOCORD. Lima. Perú.
Background and Aim: The aim of this pilot study was to evaluate the therapeutic and safety performance of an intramuscular treatment protocol of multidose of allogeneic adipose stem cells (ASCs) isolated, characterized, and expanded ex vivo from a healthy canine donor.
Materials and Methods: Twelve dogs diagnosed with canine atopic dermatitis (CAD) were intramuscularly treated with 0.5×106 of cryopreserved ASCs from a healthy immunized young canine Ehrlichia canis free donor weekly for 6 weeks. Treatment efficacy was evaluated by the pruritus index and the CAD Lesion Index (CADLI) test. Safety and adverse effects were determined by injection site reaction, weight, blood chemistry, liver function, and whole blood count.
Results: Canine ASCs obtained from a donor met the minimum qualities required for this type of cells and showed viability of 90% after thawing. The efficacy of the CADLI score and the pruritus index in 12 dogs with atopic dermatitis was statistically significant efficacy. No adverse reactions were observed at the intramuscular application site, or in relation to animal weight, blood cell populations, or liver and renal function.
Conclusion: These results suggest that intramuscular administration of cryopreserved ASCs to dogs with atopic dermatitis is a promising cellular therapeutic product for the relief of the symptoms of this disease; however, the duration of the effects obtained with this dose and with other doses should be evaluated, as well as possible immune reactions. As far as we know, this is the first report of the use of multiple intramuscular doses cryopreserved ASCs to treat atopic dermatitis. Keywords: adipose stem cells, allogeneic stem cells, canine atopic dermatitis, cellular therapy.
Keywords: adipose stem cells, allogeneic stem cells, canine atopic dermatitis, cellular therapy.
How to cite this article: Enciso N, Amiel J, Pando J, Enciso J (2019) Multidose intramuscular allogeneic adipose stem cells decrease the severity of canine atopic dermatitis: A pilot study, Veterinary World, 12(11): 1747-1754.
Received: 15-07-2019 Accepted: 30-09-2019 Published online: 08-11-2019
Corresponding author: Javier Enciso E-mail: jenciso@cientifica.edu.pe
DOI: 10.14202/vetworld.2019.1747-1754
Copyright: Enciso, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.