Vet World Vol.13 June-2020 Article-19
Research Article
Veterinary World, 13(6): 1153-1158
https://doi.org/10.14202/vetworld.2020.1153-1158
Detection of esophageal and glandular stomach calcification in cow (Bos taurus)
2. Department of Biology, College of Science, University of Al Muthanna, Al Muthanna, Iraq.
3. Department of Biology, College of Science, University of Wasit, Wasit, Iraq.
Background and Aim: The aims of this study were first to estimate calcification in the esophagus and abomasum of cows and second to quantify its appearance with increasing age using histopathological and immunohistochemical techniques.
Materials and Methods: Esophageal and abomasal samples from 24 healthy cows (Bos taurus) were collected. Hematoxylin and eosin, alizarin red, and von Kossa stains were used for histopathological analysis. Histopathological changes were confirmed with immunohistochemical staining, followed by digital image analysis.
Results: Histological findings revealed the esophagus and abomasum wall comprised four fundamental layers, the mucosa, submucosa, muscularis, and serosa or adventitia. At 1 year old, calcification was beginning to appear as fine diffused points in mucosa, submucosa, and muscularis of both esophagus and abomasum, appearing as small spots at 2 years old. With advancing age in all animals, this calcification began to appear as medium spots spread throughout all wall layers of these organs at 3 years old. By 4 years old, calcification had evolved into large dark foci spread substantially throughout the tunica submucosa and tunica muscularis. Immunohistochemical results exhibited positive immunoreaction to calcium salts in the esophagus and abomasum layers in all animals, which increased with age.
Conclusion: The current study concluded that calcification is a pathological event appearing spontaneously in various types of soft tissue, significantly increasing with age, either because of hypercalcemia and hyperphosphatemia or secondary to other diseases. Keywords: abomasum, alizarin red, calcification, esophagus, immunohistochemistry, von Kossa.
Keywords: abomasum, alizarin red, calcification, esophagus, immunohistochemistry, von Kossa.
How to cite this article: Zegyer EAK, Al Khuzaee BA, Al Badri AM (2020) Detection of esophageal and glandular stomach calcification in cow (Bos taurus), Veterinary World, 13(6): 1153-1158.
Received: 21-11-2019 Accepted: 05-05-2020 Published online: 19-06-2020
Corresponding author: Ahmed Mahdi Al Badri E-mail: ahmedalmyahi@yahoo.com
DOI: 10.14202/vetworld.2020.1153-1158
Copyright: Zegyer EAK, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.