Effects of different sterilization methods of herbal formula on phytochemical compounds and antibacterial activity against mastitis-causing bacteria

Vet World Vol.13 June-2020 Article-24

Research Article

Veterinary World, 13(6): 1187-1192

https://doi.org/10.14202/vetworld.2020.1187-1192

Dian Wahyu Harjanti¹, Fajar Wahyono¹, and Vincentia Rizke Ciptaningtyas²

1. Department of Animal Science, Faculty of Animal and Agricultural Sciences, Diponegoro University, Semarang, Central Java, Indonesia.
2. Department of Clinical Microbiology, Faculty of Medicine, Diponegoro University, Semarang, Central Java, Indonesia.

Background and Aim: The current phytobiotic industry is struggling to determine a proper sterilization method for an herbal formula that comprises multiple plant extracts. Hence, this study aims to investigate the effects of two sterilization methods of herbal formula on phytochemical compounds and antibacterial activity against mastitis-causing bacterial isolates.

Materials and Methods: The herbal formula comprised the extracts of Piper betle leaves, Curcuma domestica, and Curcuma zanthorriza. We applied two sterilization methods – sterilization using 0.45 μM syringe filter and sterilization using an autoclave at 121°C for 15 min. After sterilization, we measured phytochemical contents (phenol and flavonoid) of the herbal formula. Using the disk diffusion method, the antibacterial susceptibility test of the sterilized herbal formula against mastitis-causing bacterial isolates was conducted. Tetracycline, erythromycin, and penicillin – common antibiotics for mastitis therapy in dairy farms – were used as standard antibiotics.

Results: Compared with autoclave sterilization, syringe filter sterilization resulted in less (p<0.05) phenolic and flavonoid contents. Against Escherichia coli and Staphylococcus aureus, the filter sterilized herbal formula (E. coli: 65.9%-73%; S. aureus: 6.2%-18.1%) markedly reduced the antibacterial activity than the autoclave-sterilized herbal formula (E. coli: 2.1%- 3%; S. aureus: 4.5%-10.7%).

Conclusion: This study establishes that autoclave sterilization of the herbal formula is the best sterilization method that exerts minimal adverse effects on the phytochemical compounds and could sustain the antibacterial efficacy against mastitis-causing bacteria. Hence, the herbal formula could be used as an alternative treatment for bovine mastitis. Keywords: herbal extract, mastitis, phytobiotic, phytochemical compound, sterilization method.

Keywords: herbal extract, mastitis, phytobiotic, phytochemical compound, sterilization method.

How to cite this article: Harjanti DW, Wahyono F, Ciptaningtyas VR (2020) Effects of different sterilization methods of herbal formula on phytochemical compounds and antibacterial activity against mastitis-causing bacteria, Veterinary World, 13(6): 1187-1192.

Received: 02-01-2020 Accepted: 05-05-2020 Published online: 25-06-2020

Corresponding author: Dian Wahyu Harjanti E-mail: harjantidian@gmail.com

DOI: 10.14202/vetworld.2020.1187-1192

Copyright: Harjanti, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.