Antibiotic resistance and extended-spectrum β-lactamase in Escherichia coli isolates from imported 1-day-old chicks, ducklings, and turkey poults

Vet World Vol.13 June-2020 Article-4

Research Article

Veterinary World, 13(6): 1037-1044

https://doi.org/10.14202/vetworld.2020.1037-1044

Mona A. A. AbdelRahman¹, Heba Roshdy¹, Abdelhafez H. Samir², and Engy A. Hamed¹

1. Department of Bacteriology, Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Agriculture Research Center, P.O. Box 264, Dokki, Giza 12618, Egypt.
2. Department of Biotechnology, Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Agriculture Research Center, P.O. Box 264, Dokki, Giza 12618, Egypt.

Background and Aim: Antimicrobial resistance is a global health threat. This study investigated the prevalence of Escherichia coli in imported 1-day-old chicks, ducklings, and turkey poults.

Materials and Methods: The liver, heart, lungs, and yolk sacs of 148 imported batches of 1-day-old flocks (chicks, 45; ducklings, 63; and turkey poults, 40) were bacteriologically examined for the presence of E. coli.

Results: We isolated 38 E. coli strains from 13.5%, 6.7%, and 5.4% of imported batches of 1-day-old chicks, ducklings, and turkey poults, respectively. They were serotyped as O91, O125, O145, O78, O44, O36, O169, O124, O15, O26, and untyped in the imported chicks; O91, O119, O145, O15, O169, and untyped in the imported ducklings; and O78, O28, O29, O168, O125, O158, and O115 in the imported turkey poults. The E. coli isolates were investigated for antibiotic resistance against 16 antibiotics using the disk diffusion method and were found resistant to cefotaxime (60.5%), nalidixic acid (44.7%), tetracycline (44.7%), and trimethoprim-sulfamethoxazole (42.1%). The distribution of extended-spectrum β-lactamase (ESBL) and ampC β-lactamase genes was blaTEM (52.6%), blaSHV (28.9%), blaCTX-M (39.5%), blaOXA-1 (13.1%), and ampC (28.9%).

Conclusion: Imported 1-day-old poultry flocks may be a potential source for the dissemination of antibiotic-resistant E. coli and the ESBL genes in poultry production. Keywords: Escherichia coli, extended-spectrum β-lactamase, imported, multidrug resistance, poultry.

Keywords: Escherichia coli, extended-spectrum β-lactamase, imported, multidrug resistance, poultry.

How to cite this article: AbdelRahman MAA, Roshdy H, Samir AH, Hamed EA (2020) Antibiotic resistance and extended-spectrum β-lactamase in Escherichia coli isolates from imported 1-day-old chicks, ducklings, and turkey poults, Veterinary World, 13(6): 1037-1044.

Received: 15-11-2019 Accepted: 16-04-2020 Published online: 10-06-2020

Corresponding author: Mona A. A. AbdelRahman E-mail: drmonaali2000@yahoo.com

DOI: 10.14202/vetworld.2020.1037-1044

Copyright: AbdelRahman MAA, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.