Vet World Vol.13 June-2020 Article-5
Research Article
Veterinary World, 13(6): 1045-1049
https://doi.org/10.14202/vetworld.2020.1045-1049
Histopathological evaluation of latex of Bellaco-Caspi, Himatanthus sucuuba (Spruce) Woodson on wound healing effect in BALB/C mice
2. Department of Pharmacology, Bromatology and Toxicology, Faculty de Pharmacy and Biochemistry, Universidad Nacional Mayor de San Marcos, Lima 15001, Peru.
3. Department of Dynamic Sciences, Faculty of Medicine, Universidad Nacional Mayor de San Marcos, Lima 15001, Peru.
4. Laboratory of Pathology Clinic, Faculty of Veterinary Medicine and Zootechnics, Universidad Nacional del Altiplano, Puno 21001, Peru.
5. School of Medicine, Faculty of Health Sciences, Universidad Peruana de Ciencias Aplicadas, Lima, Peru.
Background and Aim: Himatanthus sucuuba (Spruce) Woodson (Apocynaceae) is a medicinal plant known as "Bellaco- Caspi" widely distributed in Loreto, Peru. In the Peruvian traditional medicine, the latex of the plant is used for the treatment of wounds, inflammation, ulcers, and other ailments. This study aims to evaluate the wound healing effect of the latex of H. sucuuba in BALB/C albino mice.
Materials and Methods: Thirty BALB/C male mice were used for wound healing study. In the experimental procedures, wound skin incision was performed at 2.0 cm in length until subcutaneous on the paravertebral of each animal. Under locally anesthetized with procaine cream, the treatment was conducted. All the mice were divided into three groups, the control group (A), zinc oxide cream (B), and H. sucuuba latex (C). The entire surface of the wound was treated for all the groups, and the treatments were performed daily for 15 days. The experiments were stopped on days 1, 7, and 15, respectively.
Results: The histopathological study of tissues revealed significant changes in wound healing effect in H. sucuuba latex compared to the control and B groups. Consequently, the mice treated with latex showed a significant reduction in epithelialization time and collagen formation. Furthermore, the latex showed a dose-dependent significant reduction of inflammation in the first 24 h of treatment.
Conclusion: BALB/C mice treated with the latex of H. sucuuba possess a wound healing effect that can scientifically prove the traditional use of the plant as a wound healing agent. Keywords: Apocynaceae, BALB/C mice, latex, pathology, regeneration, wound healing.
Keywords: Apocynaceae, BALB/C mice, latex, pathology, regeneration, wound healing.
How to cite this article: Calero-Armijos LL, Herrera-Calderon O, Arroyo-Acevedo JL, Rojas-Armas JP, Hañari-Quispe RD, Figueroa-Salvador L (2020) Histopathological evaluation of latex of Bellaco-Caspi, Himatanthus sucuuba (Spruce) Woodson on wound healing effect in BALB/C mice, Veterinary World, 13(6): 1045-1049.
Received: 16-03-2020 Accepted: 23-04-2020 Published online: 10-06-2020
Corresponding author: Oscar Herrera-Calderon E-mail: oherreraca@unmsm.edu.pe
DOI: 10.14202/vetworld.2020.1045-1049
Copyright: Calero-Armijos, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.