Tilapia viscera hydrolysate extract alleviates oxidative stress and renal damage in deoxycorticosterone acetate-salt-induced hypertension rats

Vet World Vol.13 November-2020 Article-26

Research Article

Veterinary World, 13(11): 2477-2483

https://doi.org/10.14202/vetworld.2020.2477-2483

Putut Har Riyadi¹, Mochammad Fitri Atho'illah², Wendy Alexander Tanod^3,4, and Irma Sarita Rahmawati⁵

1. Department of Fisheries Post Harvest Technology, Faculty of Fisheries and Marine Science, Diponegoro University, Semarang 1269, Central Java, Indonesia.
2. Department of Biology, Faculty of Mathematics and Natural Science, Brawijaya University, Malang 65145, East Java, Indonesia.
3. Department of Fisheries Product Technology, Institute of Fisheries and Marine (Sekolah Tinggi Perikanan dan Kelautan), Palu 94118, Central Sulawesi, Indonesia.
4. Department of Fisheries and Marine Science, Politeknik Negeri Nusa Utara, Tahuna 95821, North Sulawesi, Indonesia.
5. Department of Nutrition, Faculty of Medicine, Brawijaya University, Malang 65145, East Java, Indonesia.

Background and Aim: Hypertension is closely related to oxidative stress conditions, which increases malondialdehyde (MDA) expression and renal damage. Tilapia viscera hydrolysate extract (TVHE) contains compounds and peptides that act as antioxidants. This study aimed to investigate TVHE therapy effect on MDA levels and renal histological conditions in deoxycorticosterone acetate (DOCA)-salt-induced hypertension rats.

Materials and Methods: Tilapia viscera were defatted and hydrolyzed using Alcalase enzyme to obtain TVHE. TVHE antioxidant activity was measured using the 1,1-diphenyl-2-picrylhydrazyl method. Fifteen Wistar male rats were divided into five groups: Normal control (without induced DOCA-salt), DOCA-salt, DOCA-salt+Captopril 5 mg/kg body weight (BW), DOCA-salt+TVHE 150 mg/kg BW, and DOCA-salt+TVHE 300 mg/kg BW. MDA level and renal histology were observed in each group.

Results: TVHE half maximal inhibitory concentration values ranged from 3.87±0.35 μg/mL to 42.03±3.55 μg/mL, which were identified as in the very strong Blois category. TVHE and captopril therapy reduced MDA expression significantly (p<0.05) compared to DOCA-salt only. TVHE and captopril therapy also improved glomerular damage in DOCA-salt-induced hypertension rats.

Conclusion: TVHE has antioxidant ability, decreased MDA level, and decreased glomerular damage in DOCA-salt-induced hypertension rats. Keywords: antioxidant, hydrolysate, peptide, tilapia, viscera.

Keywords: antioxidant, hydrolysate, peptide, tilapia, viscera.

How to cite this article: Riyadi PH, Atho'illah MF, Tanod WA, Rahmawati IS (2020) Tilapia viscera hydrolysate extract alleviates oxidative stress and renal damage in deoxycorticosterone acetate-salt-induced hypertension rats, Veterinary World, 13(11): 2477-2483.

Received: 07-06-2020 Accepted: 08-10-2020 Published online: 23-11-2020

Corresponding author: Putut Har Riyadi E-mail: putut.riyadi@live.undip.ac.id

DOI: 10.14202/vetworld.2020.2477-2483

Copyright: Riyadi, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.