Vet World Vol.13 November-2020 Article-30
Research Article
Veterinary World, 13(11): 2508-2514
https://doi.org/10.14202/vetworld.2020.2508-2514
The wound healing action of a cream latex formulation of Jatropha gaumeri Greenm. in a pre-clinical model
2. Departamento Biotecnología, Universidad Autonoma Campeche, Mexico.
3. Centro Investigaciones Biomedicas, Universidad Autonoma Campeche, Mexico.
4. Hospital General de Especialidades, Campeche, Mexico.
Background and Aim: Jatropha gaumeri Greenm. is commonly used to treat mouth blisters and skin rashes, its latex has analgesic and anti-inflammatory activity on buccal ulcer. This study aimed to demonstrate the wound healing activity of a cream formulation of Jatropha gaumeri Greenm. latex in a murine model, provide a histological assessment of its scarring effects, and identify the family of phytochemicals involved in these effects.
Materials and Methods: Latex was obtained from the cut stalk leaves and young stems of J. gaumeri and stored in sterile tubes with protection from light. Chloroform, ethyl acetate, and aqueous fractions of the latex were obtained. Fifty male Balb/c mice aged 10-12 weeks were divided into 10 groups of five mice: Group 1 corresponded to healthy mice with wounds; Group 2 corresponded to mice with wounds and treated with A-Derma®; and from Group 3 to group 10 corresponded to mice treated with a different latex fraction. A circular skin wound of about 1 cm was made on the paravertebral region of each mouse under anesthetized and aseptic conditions. The wounds were topically treated every 24 h with the respective extracts for 22 days, after which skin tissue specimens were obtained and stained with hematoxylin-eosin and Masson's trichrome. The efficiency of healing was measured by quantifying the tensile strength of the scars. The phytochemicals in the latex were elucidated using thin chromatography.
Results: The aqueous latex fraction produced the best wound healing activity and was superior to the positive control. Reepithelialization at the histological level resulted in tissue that resembled healthy skin in terms of the appearance of collagen, the regeneration of hair follicles, and cellularity of the dermis, which showed organized epithelialization. A wound healing efficacy of 97% was observed, and it seems that alkaloids were the phytochemicals mostly likely responsible for these effects.
Conclusion: J. gaumeri latex exhibited wound healing activity, possibly mediated by phytochemicals such as alkaloids in the aqueous fraction. Keywords: alkaloids, Jatropha gaumeri latex, wound healing.
Keywords: alkaloids, Jatropha gaumeri latex, wound healing.
How to cite this article: León F, Hernandez-Zapata V, Bacab MC, Maldonado G, Lezama JA, Monteon V (2020) The wound healing action of a cream latex formulation of Jatropha gaumeri Greenm. in a pre-clinical model, Veterinary World, 13(11): 2508-2514.
Received: 13-07-2020 Accepted: 15-10-2020 Published online: 25-11-2020
Corresponding author: Victor Monteon E-mail: victormonteon@yahoo.com.mx
DOI: 10.14202/vetworld.2020.2508-2514
Copyright: León, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.