Vet World Vol.14 August-2021 Article-15
Research Article
Veterinary World, 14(8): 2097-2101
https://doi.org/10.14202/vetworld.2021.2097-2101
Immunodiagnosis of cattle fascioliasis using a 27 kDa Fasciola gigantica antigen
2. Department of Pharmaceutical Technology, Faculty of Pharmacy, Jordan University of Science and Technology, Irbid, Jordan.
3. Department of Radiology, King Abdulaziz Medical City, National Guard Hospital, Riyadh, Saudi Arabia.
4. Department of Pharmacology Faculty of Pharmacy, Mutah University, Amman, Jordan.
5. Department of Basic Medical Sciences, Faculty of Medicine, Al-Balqa Applied University, Amman, Jordan.
Background and Aim: Diagnosis of fascioliasis depends on clinical symptoms and routine laboratory tests. Recently, antibodies and circulating antigens of Fasciola were used for detecting active infections. Therefore, this study aimed to identify Fasciola gigantica antigens in the sera of infected cattle using Western blotting and enzyme-linked immunosorbent assay (ELISA) for an accurate diagnosis of cattle infected with F. gigantica.
Materials and Methods: Serum samples were obtained from 108, 23, and 19 cattle infected with Fasciola gigantica, Paramphistomum cervi, and Strongylids, respectively, including 57 non-infected cattle that were used as healthy cattle for the study. Western blotting and ELISA were then used to detect circulating Fasciola antigens at 27 kDa.
Results: The target epitope was detected in an F. gigantica adult-worm antigen preparation, excretory/secretory products, and serum from cattle infected with F. gigantica. However, it was absent in sera from P. cervi, Strongylids, and healthy cattle. The purified 27 kDa F. gigantica (FPA-27) antigen was also detected in cattle serum using ELISA with high degrees of sensitivity and specificity (94% and 82%, respectively), and the area under the receiver operating characteristic curve was 0.89 with a highly significant correlation of p<0.0001.
Conclusion: The FPA-27 is proposed to be a promising candidate for the serodiagnosis of fascioliasis in cattle. Keywords: antigen, Fasciola gigantica, fascioliasis, immunodiagnostics.
Keywords: antigen, Fasciola gigantica, fascioliasis, immunodiagnostics.
How to cite this article: Saadh MJ, Tanash SA, Almaaytah AM, Sa'adeh IJ, Aldalaen SM, Al-Hamaideh KD (2021) Immunodiagnosis of cattle fascioliasis using a 27 kDa Fasciola gigantica antigen, Veterinary World, 14(8): 2097-2101.
Received: 13-04-2021 Accepted: 28-06-2021 Published online: 16-08-2021
Corresponding author: Mohamed J. Saadh E-mail: msaadeh@meu.edu.com
DOI: 10.14202/vetworld.2021.2097-2101
Copyright: Saadh, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.