Vet World Vol.14 December-2021 Article-8
Research Article
Veterinary World, 14(12): 3126-3131
https://doi.org/10.14202/vetworld.2021.3126-3131
Detection of invA virulence gene of multidrug-resistant Salmonella species isolated from the cloacal swab of broiler chickens in Blitar district, East Java, Indonesia
2. Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya, Indonesia.
3. Department of Veterinary Public Health, Faculty of Veterinary Medicine, Wijaya Kusuma University Surabaya, Surabaya, Indonesia.
4. Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.
5. Department of Applied Microbiology, Ebonyi State University, Abakaliki, Nigeria.
Background and Aim: The increasing number of multidrug-resistant (MDR) Salmonella species on poultry farms in Indonesia has caused concern regarding human health. This study was conducted to determine the presence of the virulence gene invA in MDR Salmonella species isolated from the cloacal swab of broiler chickens in Blitar district, East Java Province, Indonesia.
Materials and Methods: Cloacal swab samples were collected by purposive sampling from 15 farms in four districts. Isolation and identification of bacteria were performed using standard microbiological techniques. Confirmation of MDR isolates was done using five different classes of antibiotics, including the beta-lactam, aminoglycoside, fluoroquinolone, phenicol, and monobactam groups. An antibiotic susceptibility test was conducted using the Kirby–Bauer disk diffusion method, and a polymerase chain reaction method was used to screen for the presence of invA.
Results: It was observed that 32.26% (50/155) of the samples were positive for Salmonella species. Of these 50 Salmonella isolates, 7 (14%) were identified as MDR strains. An important finding was the detection of invA in all the seven MDR Salmonella strains (100%) isolated from the cloacal swab of broiler chickens in Blitar district, East Java Province.
Conclusion: Veterinarians have an extremely important role in monitoring the use of antibiotics in farm animals to mitigate the rapid spread of MDR organisms in our environment, which can otherwise cause serious economic losses and also public health issues. Keywords: broiler chicken, invA gene, multidrug-resistant, public health, Salmonella.
Keywords: broiler chicken, invA gene, multidrug-resistant, public health, Salmonella.
How to cite this article: Wibisono FM, Faridah HD, Wibisono FJ, Tyasningsih W, Effendi MH, Witaningrum AM, Ugbo EN (2021) Detection of invA virulence gene of multidrug-resistant Salmonella species isolated from the cloacal swab of broiler chickens in Blitar district, East Java, Indonesia, Veterinary World, 14(12): 3126-3131.
Received: 23-06-2021 Accepted: 02-11-2021 Published online: 19-12-2021
Corresponding author: Mustofa Helmi Effendi E-mail: mheffendi@yahoo.com
DOI: 10.14202/vetworld.2021.3126-3131
Copyright: Wibisono, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.