Genetic and parasitological identification of Trypanosoma evansi infecting cattle in South Sulawesi, Indonesia

Vet World Vol.14 January-2021 Article-15

Research Article

Veterinary World, 14(1): 113-119

https://doi.org/10.14202/vetworld.2021.113-119

Agus Setiawan¹, Wisnu Nurcahyo², Dwi Priyowidodo², Rina Tri Budiati³, and Desy Sylvia Ratna Susanti³

1. Department of Animal Quarantine, Indonesia Agricultural Quarantine Agency, Makassar, Indonesia.
2. Department of Parasitology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.
3. Department of Veterinary Science, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.

Background and Aim: Sulawesi is an Indonesian island located within the Wallacea region that contains a distinctive mix of Asian and Australasian species. This distinctiveness extends to parasites, including Trypanosoma evansi, the cause of surra. Surra has non-specific clinical signs such as anemia, anorexia, weight loss, drop in milk production, and reproductive disorders which cause economic losses. Due to the trade of livestock, surra has spread in Indonesia from one island to another. The aim of this study was to investigate the trypanosomes infecting cattle in South Sulawesi, using internal transcribed spacer (ITS2) ribosomal DNA (rDNA) sequencing.

Materials and Methods: A total of 100 whole blood samples were collected from cattle in Makassar, South Sulawesi Province, Indonesia. All samples were tested using conventional parasitological methods (CPT), namely, thin blood smear, buffy coat smears, and polymerase chain reaction (PCR) testing. Positive PCR results were sequenced and phylogenetically analyzed.

Results: Only one of the 100 samples was found to be positive with microscopic observation; however, PCR analysis revealed that 3% (3/100) of samples were positive. Sequencing identified the positive samples as T. evansi, China isolate (KU552344), with a homology of 99%. Two out of three sequences showed variations in ITS2 region.

Conclusion: Based on CPT and molecular analysis, T. evansi isolates from infected cattle in South Sulawesi demonstrate genetic diversity of ITS2 sequences. Keywords: cattle, internal transcribed spacer-2, Sulawesi, Surra, Trypanosoma evansi.

Keywords: cattle, internal transcribed spacer-2, Sulawesi, Surra, Trypanosoma evansi.

How to cite this article: Setiawan A, Nurcahyo W, Priyowidodo D, Budiati RT, Susanti DSR (2021) Genetic and parasitological identification of Trypanosoma evansi infecting cattle in South Sulawesi, Indonesia, Veterinary World, 14(1): 113-119.

Received: 23-07-2020 Accepted: 04-12-2020 Published online: 15-01-2021

Corresponding author: Wisnu Nurcahyo E-mail: wisnu-nc@ugm.ac.id

DOI: 10.14202/vetworld.2021.113-119

Copyright: Setiawan, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.