Detection of multidrug-resistant Salmonella enterica subsp. enterica serovar Typhi isolated from Iraqi subjects

Vet World Vol.14 July-2021 Article-28

Research Article

Veterinary World, 14(7): 1922-1928

https://doi.org/10.14202/vetworld.2021.1922-1928

Hamzah Abdulrahman Salman¹, Ali Mohammed Abdulmohsen², Mays Noori Falih², and Zahraa Mohmoud Romi²

1. Department of Medical Laboratory Techniques, College of Medical Sciences Techniques, The University of Mashreq, Baghdad, Iraq.
2. The Biological Society of Iraq, Baghdad, Iraq.

Background and Aim: Enteric fever initiated by Salmonella enterica subsp. enterica serovar Typhi (S. Typhi) is among the most consistent disease worldwide, particularly in developing countries. The present study aimed to isolate and identify S. Typhi from typhoid suspected patients and determine their antibacterial susceptibility testing.

Materials and Methods: Thirty blood samples were collected from typhoid suspected patients in Baghdad, Iraq. The samples were cultured on SS agar and XLD agar for screening of S. Typhi. The suspected colonies were picked up and subjected to Vitek 2 compact for biochemical identification and antibacterial susceptibility testing of the organisms. Molecular identification of the isolates was performed by real-time polymerase chain reaction (RT-PCR).

Results: Black colonies were observed on cultured plates. Out of 30 samples, 27 and 29 isolates were identified as S. Typhi using Vitek 2 compact and RT-PCR, respectively. The data of the present study revealed that the strains of S. Typhi were showing multidrug resistance. All S. Typhi strains exhibited resistance to penicillins (ticarcillin and piperacillin), cephalosporins 4th G (cefepime), and monobactam (aztreonam). However, all the strains showed susceptibility against carbapenems (imipenem and meropenem) and tetracycline (minocycline).

Conclusion: RT-PCR and Vitek 2 compact showed a high level of accuracy in the detection of S. Typhi. Multidrug resistance was observed, which is an alert for the reduction of antibiotic consumption. Keywords: antibiotics susceptibility, enteric fever, multidrug-resistant, Salmonella, typhoid fever, Vitek 2 compact.

Keywords: antibiotics susceptibility, enteric fever, multidrug-resistant, Salmonella, typhoid fever, Vitek 2 compact.

How to cite this article: Salman HA, Abdulmohsen AM, Falih MN, Romi ZM (2021) Detection of multidrug-resistant Salmonella enterica subsp. enterica serovar Typhi isolated from Iraqi subjects, Veterinary World, 14(7): 1922-1928.

Received: 06-03-2021 Accepted: 18-06-2021 Published online: 26-07-2021

Corresponding author: Hamzah Abdulrahman Salman E-mail: hamza.alayash@gmail.com

DOI: 10.14202/vetworld.2021.1922-1928

Copyright: Salman, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.