Vet World Vol.14 May-2021 Article-11
Research Article
Veterinary World, 14(5): 1133-1142
https://doi.org/10.14202/vetworld.2021.1133-1142
In vitro evaluation of the probiotic potential of Lactobacillus isolated from native swine manure
2. Faculty of Technology and Community Development, Thaksin University, Phatthalung, 93210, Thailand.
Background and Aim: Using antimicrobials as a feed additive in swine production is prohibited because it is a major cause of the emergence of antimicrobial-resistant bacteria. Probiotics such as Lactobacillus spp. are an attractive alternative to reduce antimicrobial resistance and promote swine growth. This study aimed to evaluate the in vitro probiotic properties of Lactobacillus isolated from indigenous swine manure.
Materials and Methods: A total of 30 fecal samples from healthy individual indigenous pigs were collected and isolated on de Man, Rogosa, and Sharpe agar. The preliminary screen identified candidates with antibacterial activity against six pathogens and >50% survival and tolerance to acid (pH 3.0) and 1% bile salt. Isolates that passed the initial screen will be tested for other probiotic properties.
Results: Of the 314 isolates from 30 pig manure samples, 17 isolates satisfied all initial conditions for probiotic properties. Each isolate has unique, distinctive properties. Isolates B4, B5, B8, B17, B87, and B144 formed thick biofilms, whereas isolates B5, B8, and 27 adhered well to the intestinal wall and exhibited strong autoaggregation properties. Isolate B4 aggregated with Enterohemorrhagic Escherichia coli and Enteropathogenic E. coli. Tests in pH-adjusted cell-free medium indicated that the antibacterial activity resulted from bacterial acidification rather than bacteriocin formation. Sequence analysis (16S rRNA) revealed 16 of the isolates were Lactobacillus plantarum, and only one isolate was Lactobacillus salivarius.
Conclusion: We isolated 17 Lactobacillus from swine manure and demonstrated that their probiotic properties might be useful as a probiotic cocktail for swine feed. Keywords: Lactobacillus spp., native swine manure, probiotic properties.
Keywords: Lactobacillus spp., native swine manure, probiotic properties.
How to cite this article: Tuyarum C, Songsang A, Lertworapreecha M (2021) In vitro evaluation of the probiotic potential of Lactobacillus isolated from native swine manure, Veterinary World, 14(5): 1133-1142.
Received: 22-01-2021 Accepted: 22-03-2021 Published online: 11-05-2021
Corresponding author: Monthon Lertworapreecha E-mail: worapreecha@gmail.com
DOI: 10.14202/vetworld.2021.1133-1142
Copyright: Tuyarum, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.