Identification and antimicrobial susceptibility of methicillin-resistant Staphylococcus aureus-associated subclinical mastitis isolated from dairy cows in Bogor, Indonesia

Vet World Vol.14 May-2021 Article-16

Research Article

Veterinary World, 14(5): 1180-1184

https://doi.org/10.14202/vetworld.2021.1180-1184

Evi Nur Qolbaini¹, Miftahudin Majid Khoeri², Korrie Salsabila², Wisiva Tofriska Paramaiswari², Wisnu Tafroji², I. Made Artika^1,2, and Dodi Safari²

1. Department of Biochemistry, IPB University, Jl. Raya Dramaga, Bogor, 16680, Indonesia.
2. Eijkman Institute for Molecular Biology, Jl. Diponegoro No. 69 Jakarta, 10340, Indonesia.

Background and Aim: Subclinical mastitis is an udder infection devoid of clinical symptoms, and Staphylococcus aureus is one of the bacteria causing this disease. This study aimed to identify and determine the prevalence and antibiotic susceptibility of methicillin-resistant S. aureus (MRSA)-associated subclinical mastitis isolated from dairy cows in Bogor, Indonesia.

Materials and Methods: S. aureus was isolated from subclinical mastitis milk specimens. All strains were confirmed by polymerase chain reaction-based detection of staphylococcal nuc, and MRSA was confirmed by the presence of mecA. Antibiotic susceptibility was determined using the disk diffusion method.

Results: From 86 milk samples, 49 isolates (57%) were confirmed as S. aureus. All S. aureus isolates were susceptible to tetracycline, gentamicin, chloramphenicol, erythromycin, and trimethoprim/sulfamethoxazole. Nine isolates were identified as MRSA (10.5%).

Conclusion: In this study, we reported MRSA-associated subclinical mastitis in Bogor, Indonesia. Keywords: Bogor, dairy cows, methicillin-resistant Staphylococcus aureus, subclinical mastitis.

Keywords: Bogor, dairy cows, methicillin-resistant Staphylococcus aureus, subclinical mastitis.

How to cite this article: Qolbaini EN, Khoeri MM, Salsabila K, Paramaiswari WT, Tafroji W, Artika IM, Safari D (2021) Identification and antimicrobial susceptibility of methicillin-resistant Staphylococcus aureus-associated subclinical mastitis isolated from dairy cows in Bogor, Indonesia, Veterinary World, 14(5): 1180-1184.

Received: 24-10-2020 Accepted: 30-03-2021 Published online: 13-05-2021

Corresponding author: Dodi Safari E-mail: safari@eijkman.go.id

DOI: 10.14202/vetworld.2021.1180-1184

Copyright: Qolbaini, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.