Vet World Vol.14 May-2021 Article-28
Research Article
Veterinary World, 14(5): 1279-1283
https://doi.org/10.14202/vetworld.2021.1279-1283
The differences in the number of fibroblasts and blood vessels after the topical and systemic administration of Lactobacillus casei Shirota probiotics for the treatment of traumatic ulcers in Wistar rats (Rattus norvegicus)
2. Department of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
3. Department of Periodontology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
4. Postgraduate Program of Dental Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
5. Undergraduate Program of Dental Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
Background and Aim: The use of drugs as a therapy for traumatic ulcers may lead to drug resistance and other side effects. Lactobacillus casei Shirota can affect the number of fibroblasts and blood vessels in wound healing. The aim of this study was to investigate the difference in the number of fibroblast cells and blood vessels after the topical and systemic administration of L. casei Shirota probiotics in Wistar rats with traumatic ulcer.
Materials and Methods: Overall, 36 healthy male Wistar rats aged 2-3 months old and weighing 175-250 g in body weight were used as a sample. Traumatic ulcer was made on the labial fornix incisive inferior. The subject rats were divided into groups: (1) A control group over 3 days, (2) a group that used distilled water over 7 days, (3) a group that underwent topical treatment over 3 days, (4) a group that used probiotics administered topically over 7 days, (5) a group that underwent systemic treatment over 3 days, and (6) a group that took oral probiotics for the traumatic ulcers over 7 days. The number of fibroblasts and blood vessels was observed through a hematoxylin-eosin examination.
Results: Based on the results of the study, a significant difference was observed in the number of fibroblasts (p=0.00) and blood vessels (p=0.018) in the 3-day topical group that underwent a 3-day systemic administration of probiotics compared with the number of fibroblast cells in the 7-day topical group and 7-day systemic group (p=0.00).
Conclusion: Overall, significant differences were observed in the number of fibroblasts and blood vessels in Wistar rats with traumatic ulcer after undergoing the topical and systemic administration of L. casei Shirota probiotics. Keywords: blood vessel, fibroblast, Lactobacillus casei Shirota, systemic administration, topical application, traumatic ulcers.
Keywords: blood vessel, fibroblast, Lactobacillus casei Shirota, systemic administration, topical application, traumatic ulcers.
How to cite this article: Kusumaningsih T, Irmawati A, Ernawati DS, Prahasanti C, Aljunaid M, Amelia S (2021) The differences in the number of fibroblasts and blood vessels after the topical and systemic administration of Lactobacillus casei Shirota probiotics for the treatment of traumatic ulcers in Wistar rats (Rattus norvegicus), Veterinary World, 14(5): 1279-1283.
Received: 19-12-2020 Accepted: 15-03-2021 Published online: 23-05-2021
Corresponding author: Tuti Kusumaningsih E-mail: tuti-k@fkg.unair.ac.id
DOI: 10.14202/vetworld.2021.1279-1283
Copyright: Kusumaningsih, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.