Vet World   Vol.14   May-2021  Article-30

The role of Helianthus tuberosus powder in healing of full-thickness wounds in mice

Background and Aim: Recently, many medicinal plants have received considerable attention in the medical field because of their role in the wound healing potential. This study aimed to determine the effectiveness of H. tuberosus powder on the healing pathway of full-thickness cutaneous wounds in a mouse model.

Materials and Methods: H. tuberosus powder was prepared by a freeze-drying process using a lyophilizer and its active ingredients were evaluated by high-performance liquid chromatography (HPLC), while its antibacterial properties were evaluated by agar well diffusion assay. The percentage wound contraction was also assessed. Thirty mice were used, which were divided equally into two groups, a control group and a treated group. A full-thickness wound, 1 cm×1 cm in size, was established on the dorsal aspect of the thoracolumbar region, into which H. tuberosus powder was topically applied in the treated group. In contrast, the control group was left without any treatment. The animals were euthanized on days 7, 14, and 21 after wounding for histopathological study.

Results: The agar well diffusion method indicated the antibacterial activity of H. tuberosus, while the HPLC results indicated that the active ingredients of H. tuberosus powder mainly consisted of three major kinds of fatty acid. In addition, the macroscopic results of wound contraction rate and the histopathological outcomes of the healing process were significantly (p≤0.05) enhanced in the treated group compared with those in the control group.

Conclusion: H. tuberosus powder acts as an antibacterial agent with the ability to enhance the wound healing process. Keywords: antimicrobial activity, fatty acids, Jerusalem artichoke, medicinal plants, wound healing.

Keywords: antimicrobial activity, fatty acids, Jerusalem artichoke, medicinal plants, wound healing.

How to cite this article: Atiyah AG, AL-Falahi NHR (2021) The role of Helianthus tuberosus powder in healing of full-thickness wounds in mice, Veterinary World, 14(5): 1290-1298.

Received: 20-12-2020  Accepted: 08-04-2021     Published online: 24-05-2021

Corresponding author: Ali Ghazi Atiyah   E-mail: alighazivet@tu.edu.iq

DOI: 10.14202/vetworld.2021.1290-1298

Copyright: Atiyah and AL-Falahi NHR, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.