Molecular epidemiology of helminth diseases of the humpback grouper, Cromileptes altivelis, as a pattern for mapping fish diseases in the Sunda Strait, Indonesia

Vet World Vol.14 May-2021 Article-35

Research Article

Veterinary World, 14(5): 1324-1329

https://doi.org/10.14202/vetworld.2021.1324-1329

Sri Subekti¹, Muhammad Kholiqul Amiin², Hervina Benazir Ardiyanti², Muhammad Aiman Yudarana², Ivan Achmadi², and Rizhar Eman Karunia Akbar²

1. Department of Marine, Faculty of Fisheries and Marine, Universitas Airlangga, Surabaya, Indonesia.
2. Department of Biotechnology Fisheries and Marine, Universitas Airlangga, Mulyorejo, Surabaya 60115, East Java, Indonesia.

Background and Aim: Neobenedenia girellae and Haliotrema epinepheli are important but neglected helminth parasites of marine fish. The humpback grouper, the most relevant definitive host, harbors several Neobenedenia and Haliotrema spp. simultaneously on body surfaces and gills. These species can be distinguished morphologically This study aimed to identify Neobenedenia and Haliotrema spp. infestations in monogenean humpback grouper by multiplex polymerase chain reaction method, which seems to be widely distributed in the study area. Data can be used as a basis for mapping disease patterns in Strait waters.

Materials and Methods: Eighty humpback groupers (Cromileptes altivelis) were collected from eight different areas in the Sunda Strait and examined using scrapings from body surfaces and gill lamellae followed by multiplex PCR for identification.

Results: Parasites on body surfaces were recovered from 49 fish (61.2%) and were found on gill lamellae in 72 fish (90%) by microscopic examination. Endoparasites were absent. Ectoparasites identified included, N. girellae, Neobenedenia melleni eggs, Neobenedenia pasifica, Neobenedenia longiprostata, Haliotrema eukurodai, H. kurodai, Haliotrema leporinus, Haliotrema dongshaense, Haliotrema angelopterum, Haliotrema aurigae, Haliotrema scyphovagina, and H. epinepheli.

Conclusion: The distribution of trematode disease in humpback grouper in Sunda Strait waters was revealed. All parasites were from genera, Neobenedenia and Haliotrema. Risks associated with these parasites should not be overlooked. Prevention and control programs need to be extended to other marine fish. Humpback grouper should be dewormed more frequently. Keywords: humpback grouper, multiplex polymerize chain reaction, worm disease.

Keywords: humpback grouper, multiplex polymerize chain reaction, worm disease.

How to cite this article: Subekti S, Amiin MK, Ardiyanti HB, Yudarana MA, Achmadi I, Akbar REK (2021) Molecular epidemiology of helminth diseases of the humpback grouper, Cromileptes altivelis, as a pattern for mapping fish diseases in the Sunda Strait, Indonesia, Veterinary World, 14(5): 1324-1329.

Received: 17-09-2020 Accepted: 12-04-2021 Published online: 27-05-2021

Corresponding author: Muhammad Kholiqul Amiin E-mail: kholiqamiin7@gmail.com

DOI: 10.14202/vetworld.2021.1324-1329

Copyright: Subekti, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.