Efficacy of bubaline blood derived fibrin glue in silk ligature-induced acute periodontitis in Wistar rats

Vet World Vol.14 October-2021 Article-4

Research Article

Veterinary World, 14(10): 2602-2612

https://doi.org/10.14202/vetworld.2021.2602-2612

Poranee Banyatworakul¹, Nopadon Pirarat², Sujin Sirisawadi³, Thanaphum Osathanon⁴, and Chanin Kalpravidh¹

1. Department of Veterinary Surgery, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330 Thailand.
2. Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330 Thailand.
3. Biochemistry Unit, Department of Veterinary Physiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330 Thailand.
4. Dental Stem Cell Biology Research Unit, Department of Anatomy, Faculty of Dentistry, Chulalongkorn University, Bangkok, 10330 Thailand.

Background and Aim: Fibrin forms in the coagulation process, enhancing local hemostatic properties and promoting wound healing. The study aimed to evaluate the efficacy of bubaline-derived fibrin glue in silk ligature-induced periodontitis rats.

Materials and Methods: Bubaline blood–derived fibrin glue was prepared using cryoprecipitation and cryocentrifugation. Periodontitis was induced in rats by placing 5-0 silk ligatures around the mandibular first molars. The animals were divided into two groups: (1) Non-treatment and (2) bubaline fibrin glue–treated groups. Plaque, gingival inflammation, and mobility index were scored on days 1, 7, and 14 after intervention. Histological examinations were performed. The mRNA expression of inflammatory cytokines and growth factors was evaluated using a real-time polymerase chain reaction. Ligature-induced periodontitis was confirmed by the increase in inflammatory cell infiltration as well as histological bone and attachment loss.

Results: Compared to the non-treatment group, bubaline fibrin glue application reduced mononuclear cell infiltration into periodontal tissues corresponding to the reduction of collagen destruction. On days 7 and 14 after intervention, the inflammatory score and histological attachment loss were significantly lower in the bubaline fibrin glue–treated group than in the non-treatment group. A significant reduction in histological bone loss was observed in the treated group on day 7. Bubaline fibrin glue application led to a significant reduction of Tnfa and Il1b mRNA levels, while an increased expression of Pdgfa, Tgfb1, and Il10 was observed compared with the control.

Conclusion: Bubaline fibrin glue could be beneficial in periodontitis treatment aiming to reduce inflammation and delay the progression of periodontal disease. Keywords: bubaline, fibrin, inflammation, ligature-induced, periodontitis.

Keywords: bubaline, fibrin, inflammation, ligature-induced, periodontitis.

How to cite this article: Banyatworakul P, Pirarat N, Sirisawadi S, Osathanon T, Kalpravidh C (2021) Efficacy of bubaline blood derived fibrin glue in silk ligature-induced acute periodontitis in Wistar rats, Veterinary World, 14(10): 2602-2612.

Received: 07-05-2021 Accepted: 20-08-2021 Published online: 06-10-2021

Corresponding author: Nopadon Pirarat E-mail: nopadonpirarat@gmail.com

DOI: 10.14202/vetworld.2021.2602-2612

Copyright: Banyatworakul, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.