The effect of mesenchymal stem cell-conditioned medium gel on burn wound healing in rat

Vet World Vol.15 April-2022 Article-4

Research Article

Veterinary World, 15(4): 841-847

https://doi.org/10.14202/vetworld.2022.841-847

Dian Ratih Laksmitawati¹, Siti Umrah Noor², Yati Sumiyati¹, Adrian Hartanto¹, Wahyu Widowati³, and Diah Kartika Pratami⁴

1. Laboratory of Biochemistry, Faculty of Pharmacy, Universitas Pancasila, Jakarta, 12640, Indonesia.
2. Laboratory of Pharmaceutical Technology, Faculty of Pharmacy, Universitas Pancasila, Jakarta, 12640, Indonesia.
3. Medical Research Center, Faculty of Medicine, Maranatha University, Bandung, West Java, 40164, Indonesia.
4. Laboratory of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Pancasila University, Jakarta, 12640, Indonesia.

Background and Aim: Stem cells are cells that can proliferate to form a new tissue, leading to its use in regenerative therapy. Stem cells will secrete biological factors, such as growth factors, cytokines, and other proteins to their surroundings and culture medium/conditioned medium (CM), altering tissue physiology. These factors can help wound healing, but their effect on third-degree burns is poorly understood. This research aimed to study the activity of mesenchymal stem cell-conditioned medium gel in healing and repairing third-degree burns on rats skin.

Materials and Methods: Twenty-four Sprague–Dawley rats with burn wounds on the dorsal area were divided into four groups; the first group was treated with CM gel, with a concentration equivalent to 0.05% protein, the second group was treated with a placebo gel, the third group with silver sulfadiazine (SSD) cream (SSD-Burnazin contain 10 mg/g SSD), and the fourth group was not given any treatment, for 21 days, and on the final day, the rats were sacrificed, and the skins were taken. All topical treatments completely cover the wound area.

Results: Wound healing process indicators observed include wound diameter, scabs' formation, blister formation, and hair growth every day. The skins taken were processed with hematoxylin-eosin and Masson's trichrome staining. The indicators studied include neutrophil infiltration, mononuclear cell infiltration, neovascularization, collagen area, and re-epithelization ratio.

Conclusion: CM shows better wound healing than other groups and faster hair growth. Keywords: conditioned medium, mesenchymal stem cell, third-degree burn.

Keywords: conditioned medium, mesenchymal stem cell, third-degree burn.

How to cite this article: Laksmitawati DR, Noor SU, Sumiyati Y, Hartanto A, Widowati W, Pratami DK (2022) The effect of mesenchymal stem cell-conditioned medium gel on burn wound healing in rat, Veterinary World, 15(4): 841-847.

Received: 20-10-2021 Accepted: 02-03-2022 Published online: 07-04-2022

Corresponding author: Dian Ratih Laksmitawati E-mail: dian.ratih@univpancasila.ac.id

DOI: 10.14202/vetworld.2022.841-847

Copyright: Laksmitawati, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.