Wound healing activity of Tropaeolum tuberosum-based topical formulations in mice

Vet World Vol.15 February-2022 Article-20

Research Article

Veterinary World, 15(2): 390-396

https://doi.org/10.14202/vetworld.2022.390-396

Carmen R. Silva-Correa¹, Greysi I. Pazo-Medina¹, Víctor E. Villarreal-La Torre¹, Abhel A. Calderón-Peña², Cinthya L. Aspajo-Villalaz², José L. Cruzado-Razco¹, Jorge Del Rosario-Chávarri², Anabel D. González-Siccha³, Luz M. Guerrero-Espino⁴, María V. González-Blas³, William A. Sagástegui-Guarniz¹, César D. Gamarra-Sánchez¹, and Julio Hilario-Vargas⁴

1. Department of Pharmacology, School of Pharmacy and Biochemistry, National University of Trujillo, Trujillo, Perú.
2. Department of Biological Chemistry and Animal Physiology, School of Biological Science, National University of Trujillo, Trujillo, Perú.
3. Department of Biochemistry, School of Pharmacy and Biochemistry, National University of Trujillo, Trujillo, Perú.
4. Department of Physiology, School of Medicine, National University of Trujillo, Trujillo, Perú.

Background and Aim: Tropaeolum tuberosum Ruiz and Pavón, a tuber native to South America, is characterized by its antioxidant, antimicrobial, and anti-inflammatory properties that contribute to wound healing. This study aimed to evaluate the healing effect of the topical T. tuberosum formulations (gel and cream) on induced wounds in mice.

Materials and Methods: Here, an acidic ethanolic extract (1.5 N hydrochloric acid and 96% ethanol at the ratio of 15:85, v/v) was prepared with the tubers of T. tuberosum ecotype black and incorporated into topical cream and gel formulations at 1%. Thirty-twoBalb/c mice were divided into four experimental groups receiving daily topical treatments for 14 days: Group I (control; no treatment), Group II (a commercial ointment of neomycin, polymyxin B, and bacitracin), Group III (1% T. tuberosum gel), and Group IV (1% T. tuberosum cream). The wound closure in the mice was determined during the treatment; then, they were euthanized to obtain skin samples for histopathological analysis.

Results: Groups III and IV showed a higher percentage of wound closure from the 6th day of the treatment. From the 8th day, the effect was greater in Group III. The healing effect was confirmed by the histopathological changes.

Conclusion: This study concludes that the topical formulations of T. tuberosum demonstrate wound-healing activity in mice, and the most effective treatment is the 1% gel formulation. Keywords: histology, histopathological changes, skin, topical formulations, Tropaeolum tuberosum, wound healing.

Keywords: histology, histopathological changes, skin, topical formulations, Tropaeolum tuberosum, wound healing.

How to cite this article: Silva-Correa CR, Pazo-Medina GI, Villarreal-La Torre VE, Calderón-Peña AA, Aspajo-Villalaz CL, Cruzado-Razco JL, Rosario-Chávarri JD, González-Siccha AD, Guerrero-Espino LM, González-Blas MV, Sagástegui-Guarniz WA, Gamarra-Sánchez CD, Hilario-Vargas J (2022) Wound healing activity of Tropaeolum tuberosum-based topical formulations in mice, Veterinary World, 15(2): 390-396.

Received: 14-09-2021 Accepted: 13-01-2022 Published online: 19-02-2022

Corresponding author: Víctor E. Villarreal-La Torre E-mail: vvillarreal@unitru.edu.pe

DOI: 10.14202/vetworld.2022.390-396

Copyright: Silva-Correa, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.