Vet World Vol.15 January-2022 Article-15
Research Article
Veterinary World, 15(1): 119-123
https://doi.org/10.14202/vetworld.2022.119-123
Comparison of three progesterone quantification methods using blood samples drawn from bitches during the periovulatory phase
2. Clinic for Obstetrics, Gynaecology and Andrology of Large and Small Animals with Veterinary Ambulance, Justus Liebig University, Giessen, Germany.
Background and Aim: Measuring blood progesterone (P4) concentration has become an essential diagnostic tool in small animal reproductive medicine. Methods enabling precise and rapid on-site measurements are in high demand, especially for the optimization of breeding management in bitches. This study aimed to compare two commercial on-site methods (Speed™ P4, Virbac [M1] and mini VIDAS®, bioMérieux [M2]) and a well-established radioimmunoassay (RIA), which was used as a reference method.
Materials and Methods: Comparative measurements were performed on 52 blood serum samples collected from 45 clinically healthy bitches of different breeds. The dogs had been presented to determine the estrus cycle stage and predict the time of ovulation. Each sample was divided into three aliquots. In aliquot 1, P4 was measured immediately applying M2. Aliquots 2 and 3 were stored at –20°C until analysis was performed using RIA and M1. The consistency of the three methods was investigated by pairwise linear regression analyses.
Results: In RIA, the P4 concentrations ranged between 1.1 and 25.4 ng/mL. Regression analyses revealed highly significant (p<0.0001) positive correlations between the three methods applied (M1 vs. RIA: R=0.94; M2 vs. RIA: R=0.98; and M1 vs. M2: R=0.91).
Conclusion: The results show that the two commercial on-site methods tested exhibit approximately equal, high consistency with the radioimmunological reference method and can, therefore, be used beneficially in a clinical setting. However, biological interpretation of data must be performed in a method-specific manner. Keywords: dog, enzyme-linked immunofluorescence, immunochromatography, progesterone, radioimmunoassay.
Keywords: dog, enzyme-linked immunofluorescence, immunochromatography, progesterone, radioimmunoassay.
How to cite this article: Hussein HA, Schuler G, Conze T, Wehrend A (2022) Comparison of three progesterone quantification methods using blood samples drawn from bitches during the periovulatory phase, Veterinary World, 15(1): 119-123.
Received: 11-05-2021 Accepted: 17-12-2021 Published online: 24-01-2022
Corresponding author: E-mail: axel.wehrend@vetmed.uni-giessen.de
DOI: 10.14202/vetworld.2022.119-123
Copyright: Hussein, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.