Vet World Vol.15 January-2022 Article-23
Research Article
Veterinary World, 15(1): 182-187
https://doi.org/10.14202/vetworld.2022.182-187
The improvement of insulin level after hydrogen-rich water therapy in streptozotocin-induced diabetic rats
2. Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang (State University of Malang), Jl. Semarang No. 5, Malang 65145, East Java, Indonesia.
Background and Aim: Water plays a pivotal role in the body. Alteration of the fluid balance promotes metabolic disorder, thus leading to the development of various diseases, such as diabetes mellitus (DM). Hydrogen-rich water (HW) is recognized as a novel antioxidant. This study aimed to investigate the role of HW on insulin, insulin receptor (IRs), and superoxide dismutase (SOD) levels in streptozotocin (STZ)-induced diabetic rats.
Materials and Methods: A total of 30 male Wistar rats were randomly divided into five groups: Normal (N), DM rats, DM+metformin (DM+Met, 45 mg/kg body weight [BW]), DM+Met+HW, and DM+HW. DM rats were induced by feeding them a high-fat diet for 30 days and then injecting with repeated low doses of STZ (35 mg/kg BW) intraperitoneally. Fresh HW was administered orally and ad libitum for 14 days. Insulin, IRs, and SOD were observed in each group.
Results: HW therapy increased the level and expression of insulin and IRs. In addition, treatment with HW also elevated the SOD levels in the serum and liver. The study results indicated no significant differences between the administration of HW and metformin.
Conclusion: HW has antioxidant activity in STZ-induced DM rats, increasing insulin, IRs, and SOD. Keywords: diabetes mellitus, hydrogen-rich water, insulin receptor, insulin, superoxide dismutase.
Keywords: diabetes mellitus, hydrogen-rich water, insulin receptor, insulin, superoxide dismutase.
How to cite this article: Retnaningtyas E, Susatia B, Arifah SN, Lestari SR (2022) The improvement of insulin level after hydrogen-rich water therapy in streptozotocin-induced diabetic rats, Veterinary World, 15(1): 182-187.
Received: 29-09-2021 Accepted: 28-12-2021 Published online: 28-01-2022
Corresponding author: E-mail: ekowati_retnaningtyas@poltekkes-malang.ac.id
DOI: 10.14202/vetworld.2022.182-187
Copyright: Retnaningtyas, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.