Association of natural resistance-associated macrophage protein 1 polymorphisms with Salmonella fecal shedding and hematological traits in pigs

Vet World Vol.15 November-2022 Article-26

Research Article

Veterinary World, 15(11): 2738-2743

https://doi.org/10.14202/vetworld.2022.2738-2743

Nattariga Suwannawong^1,2, Uschara Thumarat³, and Pitchayanipa Phongphanich^1,2

1. Animal Production Innovation and Management Division, Faculty of Natural Resources, Prince of Songkla University, Songkhla 90112, Thailand.
2. Center of Excellence on Agricultural Biotechnology (AG-BIO/MHESI), Bangkok 10900, Thailand.
3. Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Songkhla 90112, Thailand.

Background and Aim: Natural resistance-associated macrophage protein 1 encoding gene (Nramp1) plays a role in immune response and disease resistance. This study aimed to investigate the polymorphisms of Nramp1 intron 6 concerning Salmonella shedding and hematological traits in pigs.

Materials and Methods: A total of 40 commercial pigs (three-way Large White x Landrace x Duroc cross) were genotyped using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method and analyze the relationship between the polymorphisms of the Nramp1 gene and Salmonella fecal shedding and hematological parameters.

Results: Nramp1 was shown to be polymorphic in these pigs. The Nramp1 gene has two alleles (A and B) and two genotypes (AB and BB). The BB genotype had a higher frequency than the AB genotype. A significant relationship between the BB genotype and the number of Salmonella in feces compared to the AB genotype (p < 0.05) on 7 days post-inoculation (DPI) was revealed in the association analysis. The single-nucleotide polymorphism at intron 6 in the Nramp1 gene was linked to white and red blood cells 2 and 7 DPI (p < 0.05).

Conclusion: The Nramp1 gene was suggested by these findings to be potentially used as a molecular marker for the genetic selection of disease susceptibility in pig breeding. Keywords: hematological trait, Nramp1, Salmonella shedding pig.

Keywords: hematological trait, Nramp1, Salmonella shedding pig.

How to cite this article: Suwannawong N, Thumarat U, and Phongphanich P (2022) Association of natural resistance-associated macrophage protein 1 polymorphisms with Salmonella fecal shedding and hematological traits in pigs, Veterinary World, 15(11): 2738–2743.

Received: 12-08-2022 Accepted: 17-10-2022 Published online: 29-11-2022

Corresponding author: Pitchayanipa Phongphanich E-mail: pitchayanipa.k@psu.ac.th

DOI: 10.14202/vetworld.2022.2738-2743

Copyright: Suwannawong, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.