Vet World Vol.15 October-2022 Article-17
Research Article
Veterinary World, 15(10): 2506-2510
https://doi.org/10.14202/vetworld.2022.2506-2510
Molecular diagnosis of bovine tuberculosis on postmortem carcasses during routine meat inspection in Benin: GeneXpert® testing to improve diagnostic scheme
2. National Center of TB Control, Cotonou, Benin.
3. Virology Division, National Veterinary Research Institute, PMB 0001, Vom, Nigeria.
Background and Aim: Bovine tuberculosis (TB) is a zoonotic disease of major public health importance, particularly in African countries, where control measures are limited or largely not applied. This study aimed to determine the accuracy of the currently used bovine TB diagnostic method at slaughterhouses in Benin; this is to contribute to the betterment and improvement in the epidemiological surveillance of the disease in the country.
Materials and Methods: A total of 40 tissue samples were collected from meat/viscera (lung, liver, heart, kidney, and the gastro-intestinal tract tissues) at Cotonou slaughterhouses from ruminants suspected to be infected with bovine TB during routine meat inspection. The collected samples were analyzed using GeneXpert testing technique as a reference method.
Results: Twenty-six samples tested positive out of the 40 suspected tissue samples collected by GeneXpert diagnostic technique; this shows the limitation of the routine meat inspection in detecting bovine TB as currently performed in Benin.
Conclusion: The outcome of the use of the molecular technique, therefore, supports the importance of the use of a molecular tool alongside the routine meat inspection for a better understanding of the epidemiology of bovine TB in Benin. However, more robust technical and policy efforts are needed for a sustainable implementation of such a strategy. Keywords: genomic amplification, non-tuberculosis mycobacteria, one health, polymerase chain reaction accuracy, zoonotic tuberculosis.
Keywords: genomic amplification, non-tuberculosis mycobacteria, one health, polymerase chain reaction accuracy, zoonotic tuberculosis.
How to cite this article: Boko CK, Zoclanclounon AR, Adoligbe CM, Dedehouanou H, M'Po M, Mantip S, and Farougou S (2022) Molecular diagnosis of bovine tuberculosis on postmortem carcasses during routine meat inspection in Benin: GeneXpert
Received: 30-03-2022 Accepted: 27-09-2022 Published online: 31-10-2022
Corresponding author: Ange-Régis Zoclanclounon E-mail: zocmanregis@gmail.com
DOI: 10.14202/vetworld.2022.2506-2510
Copyright: Boko, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.