Research (Published online: 07-05-2023)
5. Effects of ultrasound-induced stress on gut microbiota of mice
Irina Chernukha, Ekaterina Vasilevskaya, Ksenia Klimina, Roman Yunes, Nadezhda Kupaeva, Galina Tolmacheva, Anastasiya Kibitkina, Valery Danilenko, Sergey Karabanov, and Liliya Fedulova
Veterinary World, 16(5): 929-938
Irina Chernukha: Department of Experimental Clinic and Research Laboratory for Bioactive Substances of Animal Origin, V.M. Gorbatov Federal Research Center for Food Systems, Moscow, Russia.
Ekaterina Vasilevskaya: Department of Experimental Clinic and Research Laboratory for Bioactive Substances of Animal Origin, V.M. Gorbatov Federal Research Center for Food Systems, Moscow, Russia.
Ksenia Klimina: Department of Genetics of Microorganisms, Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, Russia.
Roman Yunes: Department of Genetics of Microorganisms, Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, Russia.
Nadezhda Kupaeva: Department of Experimental Clinic and Research Laboratory for Bioactive Substances of Animal Origin, V.M. Gorbatov Federal Research Center for Food Systems, Moscow, Russia.
Galina Tolmacheva: Department of Experimental Clinic and Research Laboratory for Bioactive Substances of Animal Origin, V.M. Gorbatov Federal Research Center for Food Systems, Moscow, Russia.
Anastasiya Kibitkina: Department of Experimental Clinic and Research Laboratory for Bioactive Substances of Animal Origin, V.M. Gorbatov Federal Research Center for Food Systems, Moscow, Russia.
Valery Danilenko: Department of Genetics of Microorganisms, Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, Russia.
Sergey Karabanov: Department of Experimental Clinic and Research Laboratory for Bioactive Substances of Animal Origin, V.M. Gorbatov Federal Research Center for Food Systems, Moscow, Russia.
Liliya Fedulova: Department of Experimental Clinic and Research Laboratory for Bioactive Substances of Animal Origin, V.M. Gorbatov Federal Research Center for Food Systems, Moscow, Russia.
doi: 10.14202/vetworld.2023.929-938
Article history: Received: 16-12-2022, Accepted: 10-04-2023, Published online: 07-05-2023
Corresponding authors: Sergey Karabanov
E-mail: karabans89@gmail.com
Citation: Chernukha I, Vasilevskaya E, Klimina K, Yunes R, Kupaeva N, Tolmacheva G, Kibitkina A, Danilenko V, Karabanov S, and Fedulova L (2023) Effects of ultrasound-induced stress on gut microbiota of mice, Veterinary World, 16(5):929-938.
Abstract
Background and Aim: Prolonged stress causes deleterious effects on both the organism and its microbiota. In this study, we examined the effects of exposure to variable frequency ultrasound (US) on the gut microbiota-liver-brain axis of mice.
Materials and Methods: This study was conducted on 20 mature clinically healthy sexually naive C57BL/6J male mice (42–45 days old). Group 1 (Normal) consisted of healthy intact mice (n = 10). Group 2 (Stress) consisted of mice subjected to US-induced stress (n = 10) for 20 days with alternating frequencies (20–45 kHz). Stool samples were collected on days 0, 10, and 20, and the corresponding DNA was later subjected to 16SrRNA sequencing. After mice were sacrificed on day 21, the leukocyte count, blood serum biochemical parameters, and liver and brain antioxidant status were measured. Behavioral testing was performed on days 17, 18, and 19.
Results: Ultrasound lead to higher stress and anxiety levels; increase in creatinine by 8.29% and gamma-glutamyltransferase activity by 5 times, a decrease in alkaline phosphatase activity by 38.23%, increase of de Ritis coefficient by 21.34%; increased liver and brain superoxide dismutase level by 20.8% and 21.5%, respectively; the stress-related changes in the gut microbiota composition – Bacteroidaceae and Firmicutes.
Conclusion: Subjecting mice to 20 days of US-induced stress leads to systemic disorders due to oxidative stress and a decrease in the diversity of the gut microbiota.
Keywords: antioxidant system, bacterial metabolites, gut microbiota, ultrasound-induced stress.