A rabbit model of ear otitis established using the Malassezia pachydermatis strain C23 from dogs

Vet World Vol.16 November-2023 Article-2

Research Article

Veterinary World, 16(11): 2192-2199

https://doi.org/10.14202/vetworld.2023.2192-2199

Nadezhda Sachivkina^1,2, Arfenya Karamyan², Olesya Petrukhina², Olga Kuznetsova³, Ekaterina Neborak³, and Alfia Ibragimova^4,5

1. Department of Microbiology V.S. Kiktenko, Institute of Medicine, Peoples’ Friendship University of Russia (RUDN University), Moscow, Russia.
2. Department of Veterinary Medicine, Agrarian Technological Institute, Peoples’ Friendship University of Russia (RUDN University), Moscow, Russia.
3. Department of Biochemistry T.T. Berezov, Institute of Medicine, Peoples’ Friendship University of Russia (RUDN University), Moscow, Russia.
4. Department of Foreign Languages, Institute of Medicine, Peoples’ Friendship University of Russia (RUDN University), Moscow, Russia.
5. Department of General Pharmaceutical and Biomedical Technologies, Institute of Medicine, Peoples’ Friendship University of Russia (RUDN University), Moscow, Russia.

Background and Aim: Fungal infections are a growing problem for both humans and animals due to the emergence of pathogenic strains resistant to modern antifungal treatments. To evaluate the efficacy of new antifungal drugs, it is essential to develop animal models that demonstrate typical responses to both the infection (pathogenesis and clinical course) and to the treatment, including adverse effects. In this study, we established a rabbit otitis model by infection of an aggressive multidrug-resistant strain from dogs, Malassezia pachydermatis C23, with no need for concomitant immunosuppression.

Materials and Methods: Twenty healthy adult male gray giant rabbits (1 year old, 5.5 kg) were inoculated once with M. pachydermatis C23 at 108 colony-forming units/mL. We observed the clinical signs of the disease and collected ear smears and blood samples every 5 days.

Results: The infection progressed rapidly and exhibited characteristic clinical signs without spontaneous recovery for at least 1 month. In fact, substantial deterioration was observed as evidenced by blood parameters.

Conclusion: This rabbit otitis model established using an aggressive drug-resistant fungus strain without immunosuppression could prove valuable for testing novel antifungal agents. Keywords: in vivo model, Malassezia pachydermatis, otitis media, rabbit.

Keywords: in vivo model, Malassezia pachydermatis, otitis media, rabbit.

How to cite this article: Sachivkina N, Karamyan A, Petrukhina O, Kuznetsova O, Neborak E, and Ibragimova A (2023) A rabbit model of ear otitis established using the Malassezia pachydermatis strain C23 from dogs, Veterinary World, 16(11): 2192-2199.

Received: 08-07-2023 Accepted: 04-10-2023 Published online: 01-11-2023

Corresponding author: Nadezhda Sachivkina E-mail: sachivkina@yandex.ru

DOI: 10.14202/vetworld.2023.2192-2199

Copyright: Sachivkina, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.