Stability of African swine fever virus genome under different environmental conditions

Vet World Vol.16 November-2023 Article-25

Research Article

Veterinary World, 16(11): 2374-2381

https://doi.org/10.14202/vetworld.2023.2374-2381

Wei Zheng, Jiahui Xi, Yin Zi, Jinling Wang, Yue Chi, Min Chen, Qingjian Zou, Chengcheng Tang, and Xiaoqing Zhou

Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, South China Institute of Large Animal Models for Biomedicine, School of Biotechnology and Health Science, Wuyi University, Jiangmen, 529000, China.

Background and Aim: African swine fever (ASF), a globally transmitted viral disease caused by ASF virus (ASFV), can severely damage the global trade economy. Laboratory diagnostic methods, including pathogen and serological detection techniques, are currently used to monitor and control ASF. Because the large double-stranded DNA genome of the mature virus particle is wrapped in a membrane, the stability of ASFV and its genome is maintained in most natural environments. This study aimed to investigate the stability of ASFV under different environmental conditions from both genomic and antibody perspectives, and to provide a theoretical basis for the prevention and elimination of ASFV.

Materials and Methods: In this study, we used quantitative real-time polymerase chain reaction for pathogen assays and enzyme-linked immunosorbent assay for serological assays to examine the stability of the ASFV genome and antibody, respectively, under different environmental conditions.

Results: The stability of the ASFV genome and antibody under high-temperature conditions depended on the treatment time. In the pH test, the ASFV genome and antibody remained stable in both acidic and alkaline environments. Disinfection tests revealed that the ASFV genome and antibody were susceptible to standard disinfection methods.

Conclusion: Collectively, the results demonstrated that the ASFV genome is highly stable in favorable environments but are also susceptible to standard disinfection methods. This study focuses on the stability of the ASFV genome under different conditions and provides various standard disinfection methods for the prevention and control of ASF. Keywords: African swine fever, enzyme-linked immunosorbent assay, quantitative polymerase chain reaction, standard disinfection methods, virus stability.

Keywords: African swine fever, enzyme-linked immunosorbent assay, quantitative polymerase chain reaction, standard disinfection methods, virus stability.

How to cite this article: Zheng W, Xi J, Zi Y, Wang J, Chi Y, Chen M, Zou Q, Tang C, and Zhou X (2023) Stability of African swine fever virus genome under different environmental conditions, Veterinary World, 16(11): 2374-2381.

Received: 09-08-2023 Accepted: 25-10-2023 Published online: 27-11-2023

Corresponding author: E-mail:

DOI: 10.14202/vetworld.2023.2374-2381

Copyright: Zheng, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.