Vet World Vol.17 April Article - 10
Review Article
Veterinary World, 17(4): 811-819
https://doi.org/10.14202/vetworld.2024.811-819
Indirect hemagglutination assay for diagnosing brucellosis: Past, present, and future
1 Laboratory of Infectious Pathology of Farm Animals, Caspian Zonal Research Veterinary Institute, Makhachkala, Dagestan, Russia.
2 Department of Microbiology and Biotechnology, Faculty of Veterinary and Livestock Technology, S. Seifullin Kazakh Agrotechnical Research University, Astana, Kazakhstan.
Brucellosis is a zoonotic disease that causes enormous losses in livestock production worldwide and has a significant public health impact. None of the brucellosis-free countries is currently able to guarantee their ability to prevent the introduction of the pathogen due to the increase in tourism and the expansion of migration. The timely identification of infected animals is an effective means of preventing brucellosis and minimizing the epidemiological risk. The tube agglutination test, Rose Bengal plate test, complement fixation test, and enzyme-linked immunosorbent assay, which are routinely used to identify seropositive productive animals, have limitations and results that do not always correlate. The indirect hemagglutination assay (IHA) stands out among non-traditional methods because it is affordable, has a simple protocol, and is more reliable than classical serological tests, especially in cases of questionable and/or false-negative results. The diagnostic value of the IHA has long been studied by laboratories in several countries, but mostly by post-soviet research teams; therefore, the results continue to be published in Russian-language journals, ensuring that the local scientific community can access the results. In addition, the efficacy of this test for the diagnosis of brucellosis and other infectious diseases has not yet been reviewed. The purpose of this review was to summarize the results of studies on the development and use of IHA for the diagnosis of brucellosis and to determine the prospects for further improvement
Keywords: brucellosis, diagnostic value, indirect hemagglutination assay, prospects for improving the test, serological tests.
How to cite this article: Mikailov MM, Gunashev SA, Yanikova EA, Halikov AA, and Bulashev AK (2024) Indirect hemagglutination assay for diagnosing brucellosis: Past, present, and future, Veterinary World, 17(4): 811-819.
Received: 07-02-2023 Accepted: 21-03-2024 Published online: 15-04-2024
Corresponding author: E-mail:
DOI: 10.14202/vetworld.2024.811-819
Copyright: Mikailov, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/ by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/ publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
