Vet World   Vol.18   November-2025  Article - 21 

Synergistic effects of Lactiplantibacillus plantarum and Acacia mangium leaf tannin extract on the fermentation quality, digestibility, and metabolomic profile of Indigofera silage

Farisha Rachma Azzahra¹ , Irwan Susanto¹ , Nahrowi Nahrowi^2,3 , Rohmatussolihat Rohmatussolihat⁴ , Rusli Fidriyanto⁴ , Yantyati Widyastuti⁵ , Yulianri Rizki Yanza⁶ , Vincent Niderkorn⁷ , Roni Ridwan⁴ , and Anuraga Jayanegara²

Background and Aim: Indigofera zollingeriana is a high-protein tropical legume with potential as a sustainable ruminant feed; however, its ensiling is challenged by rapid proteolysis and ammonia accumulation. Incorporating lactic acid bacteria (LAB) inoculants and natural tannin sources may enhance fermentation quality and nitrogen preservation. This study evaluated the effects of Lactiplantibacillus plantarum inoculant and Acacia mangium leaf tannin extract, individually and in combination, on the chemical composition, ensiling characteristics, in vitro rumen fermentation, digestibility, and metabolomic profiles of Indigofera silage.

Materials and Methods: A completely randomized design was used with four treatments: (R0) control, (R1) L. plantarum (10⁶ colony forming units/g FM), (R2) 1% A. mangium extract, and (R3) combination of both additives, each with five replicates. Silages were fermented anaerobically for 30 days. Analyses included proximate composition, pH, lactic acid, ammonia nitrogen (NH3-N), in vitro gas and methane (CH4) production, volatile fatty acids (VFA), digestibility, and untargeted metabolomics of both silage and rumen fluid using gas chromatography–mass spectrometry. Data were evaluated using a one-way analysis of variance, Duncan’s test, principal component analysis, and partial least squares discriminant analysis.

Results: L. plantarum lowered (p < 0.05) silage pH and fiber fractions but increased NH3-N due to enhanced deamination. A. mangium tannins effectively suppressed proteolysis, reducing NH3-N by 11.85%. Their combination improved (p < 0.05) dry- and organic-matter digestibility (↑ ≈ 9%), increased the propionate proportion by 6.82%, and lowered the acetate-to-propionate ratio, indicating a shift toward more energy-efficient rumen fermentation without significant methane (CH4) inhibition. Metabolomic profiling identified 23 key metabolites in silage and 11 in rumen fluid; acacia tannins increased fatty acyl compounds (+14.3%) while LAB enhanced prenol lipids, reflecting modified lipid and oxygen-derived pathways that improve nutrient utilization.

Conclusion: The combination of L. plantarum and A. mangium leaf extract synergistically improved Indigofera silage quality, nutrient preservation, and digestibility while modulating beneficial metabolites associated with rumen fermentation efficiency. This integrated additive strategy represents a sustainable and locally adaptable approach for tropical ruminant feed production.

Keywords: Acacia mangium, digestibility, Indigofera zollingeriana, Lactiplantibacillus plantarum, metabolomics, silage fermentation, tannins.

How to cite this article: Azzahra FR, Susanto I, Nahrowi N, Rohmatussolihat R, Fidriyanto R, Widyastuti Y, Yanza YR, Niderkorn V, Ridwan R, and Jayanegara A (2025) Synergistic effects of Lactiplantibacillus plantarum and Acacia mangium leaf tannin extract on the fermentation quality, digestibility, and metabolomic profile of Indigofera silage, Veterinary World, 18(11): 3571-3593.

Received: 07-07-2025   Accepted: 26-10-2025   Published online: 27-11-2025

Corresponding author: Roni Ridwan and Anuraga Jayanegara    E-mail: roni001@brin.go.id and anuraga.jayanegara@gmail.com

DOI: 10.14202/vetworld.2025.3571-3593

Copyright: Azzahra, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.