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Research
6. Influence
of serum and hormones on in
vitro maturation of
buffalo oocytes -
S. A. Adlak, K. P. Khillare, C. H. Pawshe and S.W. Mude
Vet World. 2008; 1(8): 243-244
Abstract
The
influence of different combinations of protein supplements in the
presence of different concentrations of commercially available FSH
(FSH Folltropin-V) and steroids (17-ß oestradiol) were studied in
order to establish optimal condition for in
vitro maturation of
buffalo oocytes. The isolated 10 immature oocytes cultured in
Ham’s F-10 + sera (buffalo oestrus serum or fetal bovine serum) +
different concentrations of FSH i.e. 1, 10 and 20 µg/ml and
oestradiol at 39oC
in 5% CO2in
air and 95% relative humidity and incubated for 24 hrs. The
oocytes matured in presence of 10% BES and 10% FBS showed higher
maturation rate than the medium alone. The addition of different
concentrations of FSH and oestradiol (E2) in the presence of serum
to medium showed improved maturation rate over that serum alone.
However higher maturation rate (71.05%) was observed in Ham’s F-10
+ FBS + E2 + 10 µg/ml FSH than Ham’s F-10 + BES + E2 + 10 µg/ml
FSH i.e. 51.16%, but no significant differences was observed. In
the present study, FBS and 10 µg/ml concentration of commercially
available crude FSH in the presence of oestradiol and suitable
protein supplement found to be most suitable medium for maturation
of buffalo oocytes.
Keywords :
Nuclear maturation, fetal bovine serum, germinal vesicle,
metaphase.
