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Research (Published online: 19-02-2017)

13. Attribution of antibacterial and antioxidant activity of Cassia tora extract toward its growth promoting effect in broiler birds - Jyoti Sahu, K. M. Koley and B. D. Sahu

Veterinary World, 10(2): 221-226

 

 

   doi: 10.14202/vetworld.2017.221-226

 

Jyoti Sahu: Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science and Animal Husbandry, Anjora, Durg - 491 001, Chhattisgarh, India.

K. M. Koley: Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science and Animal Husbandry, Anjora, Durg - 491 001, Chhattisgarh, India.

B. D. Sahu: Department of Livestock Development, Dondi, Balod - 491 226, Chhattisgarh, India.

 

Received: 25-09-2016, Accepted: 07-01-2017, Published online: 19-02-2017

 

Corresponding author: Jyoti Sahu, e-mail: dr.jyotisahu@gmail.com


Citation: Sahu J, Koley KM, Sahu BD (2017) Attribution of antibacterial and antioxidant activity of Cassia tora extract toward its growth promoting effect in broiler birds, Veterinary World, 10(2): 221-226.



Aim: The study was conducted to evaluate the attribution of antibacterial and antioxidant activity of methanolic extract of Cassia tora toward its growth promoting effect in broiler birds.

Materials and Methods: A limit test was conducted for C. tora extract in Wistar albino rats. Phytochemical screening of methanolic extract of leaves of C. tora was carried out. In-vitro antibacterial activity was measured by disc diffusion method. 1-day-old Ven Cobb broiler birds (n=90) were randomly allocated into three groups consisting of three replicates with 10 birds in each group. The birds of group T1 (Control) received basal diet, whereas birds of group T2 (Standard) received an antibiotic (Lincomycin at 0.05% in feed). The birds of group T3 (Test) received Cassia tora extract (CSE) at 0.4 g/L in drinking water in addition to basal diet. The treatment was given to birds of all the groups for 6 weeks. Antioxidant activity of C. tora was determined in blood of broiler birds. Cumulative body weight gain, feed intake, feed conversion ratio (FCR), dressing percent, and organ weight factor were evaluated to determine growth performance in broiler birds.

Results: Phytochemicals in C. tora were screened. Sensitivity to Escherichia coli and resistant to Staphylococcus aureus and Pseudomonas aeruginosa was observed in in-vitro antibacterial activity test. At the end of 6th week, antioxidant activity reflected significantly (p≤0.05) lower level of erythrocyte malondialdehyde and higher levels of reduced glutathione (GSH) and GSH peroxidase in broiler birds of group T2 and T3 as compared to broiler of group T1. Mean cumulative body weight gain of birds of T2 and T3 were significantly (p≤0.05) higher as compared to T1. Mean FCR of birds of group T3 decreased significantly than group T1.

Conclusion: Supplementation of C. tora leaves extract at 0.4 g/L in drinking water improved growth performance in broiler birds due to its antimicrobial and antioxidant activity. Therefore, it could be used as an alternative to antibiotic growth promoter in poultry ration.

Keywords: antibacterial activity, antioxidant activity, broiler birds, Cassia tora, growth promoter.



1. Vision 2030, Project Directorate on Poultry. (2011) Available from: http://www.pdonpoultry.org/pdpnew/images/extfiles/reports/PDP_Vision_2030.pdf. Accessed on 27-02-2016.
 
2. McNamee, S.E., Cunningham, R. and Elliott, C.T. (2013) Simultaneous immunochemical detection of four banned antibiotic growth promoters in raw and cooked poultry tissue. Food Addit. Contam. A., 30: 1270-1278.
https://doi.org/10.1080/19440049.2013.801087
PMid:23789918
 
3. Shakywar, Y., Jain, A., Verma, M., Panwar, A.S. and Agarwal, A. (2011) Pharmacognostical properties and their traditional uses of Cassia tora. Int. J. Pharm. Biol. Arch., 2(5): 1311-1318.
 
4. Shaikh, R. and Syed, I.Z. (2015) Proximate and phytochemical analysis of Cassia tora leaves. J. Res. Pharm. Sci., 2(8): 1-3.
 
5. OECD. (2001) Acute oral toxicity - Up and down procedure. OECD Guidelines for Testing of Chemicals. Guideline 425. OECD, Paris. p1-26.
 
6. Raaman, N. (2006) Phytochemical Techniques. New India Publishing Agency, Pitam Pura, New Delhi. p9-24.
 
7. Andrews, J.M. (2001) BSAC working party on susceptibility testing ft. BSAC standardized disc susceptibility testing method. J. Antimicrob. Chemother., 4: 43-57.
https://doi.org/10.1093/jac/48.suppl_1.43
 
8. Placer, Z.A., Cushman, L. and Johnon, B. (1966) Estimation of products of lipid peroxidation (malonldialdehyde) in biochemical system. Anal. Biochem., 16: 359-364.
https://doi.org/10.1016/0003-2697(66)90167-9
 
9. Prins, H.K. and Loos, J.A. (1969) Biochemical Methods in Red Cell Genetics. Academic Press, London. p127-129.
 
10. Paglia, D.E. and Valentine, W.N. (1967) Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J. Lab. Clin. Med., 70: 158-159.
PMid:6066618
 
11. Sang-Oh, P., Chae-Min, R., Buyng-Sung, P. and Jong, H. (2013) The meet quality and growth performance in broiler chickens fed diet with Cinnamon powder. J. Environ. Biol., 34: 127-133.
PMid:24006819
 
12. Hashemi, S.R. and Davoodi, H. (2011) Herbal plants and their derivatives as growth and health promoters in animal nutrition. Vet. Res. Commun., 35: 169-180.
https://doi.org/10.1007/s11259-010-9458-2
PMid:21213046
 
13. Dhama, K., Tiwari, R., Khan, R.U., Chakraborty, S., Gopi, M., Karthik, K., Saminathan, M., Desingu, P.A. and Sunkara, L.T. (2014) Growth promoters and novel feed additives improving poultry production and health, bioactive principles and beneficial applications: The trends and advances - A review. Int. J. Pharmacol., 10: 129-159.
https://doi.org/10.3923/ijp.2014.129.159
 
14. Kumar, P., Kumar, S., Tripathi, M.K., Mehta, N., Ranjan, R., Bhat, Z.F. and Singh, P.K. (2013) Flavonoids in the development of functional meat products: A review. Vet. World, 6(8): 573-578.
https://doi.org/10.5455/vetworld.2013.573-578
 
15. Hendra, R., Ahmad, S., Sukari, A., Shukor, M.Y. and Oskoueian, E. (2001) Flavonoid analyses and antimicrobial activity of various parts of Phaleria macrocarpa (Scheff.) Boerl fruit. Int. J. Mol. Sci.,12: 3422-3431.
https://doi.org/10.3390/ijms12063422
PMid:21747685 PMCid:PMC3131569
 
16. Saxena, M., Saxena, J., Nema, R., Singh, D. and Gupta, A. (2013) Phytochemistry of medicinal plants. J. Pharmacogn. Phytochem., 1(6): 168-182.
 
17. Lee, N.H., Lee, S.M., Song, D.H., Yang, J.Y. and Lee, H.S. (2013) Antimicrobial effect of emodin isolated from Cassia tora seeds against food-borne bacteria. J. Appl. Biol. Chem. 56(3): 187-189.
https://doi.org/10.3839/jabc.2013.030
 
18. Chichilichi, B., Mohanty, G.P., Mishra, S.K., Pradhan, C.R., Behura, N.C., Das, A. and Behera, K. (2015) Effect of partial supplementation of sun-dried Azolla as a protein source on the immunity and antioxidant status of commercial broilers. Vet. World, 8(9): 1126-1130.
https://doi.org/10.14202/vetworld.2015.1126-1130
PMid:27047208 PMCid:PMC4774782
 
19. Sirappuselvi, S. and Chitra, M. (2012) In-vitro antioxidant activity of Cassia tora. Int. Res. J. Biol. Sci. 1(6): 57-61.
 
20. Sugiyama, T., Nagata, J., Yamagishi, A., Endoh, K., Saito, M., Yamada, K., Yamada, S. and Umegaki, K. (2006) Selective protection of curcumin against CCl4-induced inactivation of hepatic cytochrome P450 isozymes in rats. Life Sci., 78: 2188-2193.
https://doi.org/10.1016/j.lfs.2005.09.025
PMid:16288784
 
21. Kirubakaran, A., Moorthy, M., Chitra, R. and Prabakar, G. (2016) Influence of combinations of fenugreek, garlic, and black pepper powder on production traits of the broilers. Vet. World, 9(5): 470-474.
https://doi.org/10.14202/vetworld.2016.470-474
PMid:27284222 PMCid:PMC4893717
 
22. Ayssiwede, S.B., Chrysostome, C., Ossebil, W., Dieng, A., Hornick, J.L. and Missohou, A. (2010) Digestibility and metabolic utilisation and nutritional value of Cassia tora (Linn.) Leaves meal incorporated in the diets of indigenous Senegal chickens. Rev. Med. Vet. Toulouse, 161(12): 549-558.
 
23. Saiyed, M.A., Joshi, R.S., Savaliya, F.P., Patel, A.B., Mishra, R.K. and Bhagora, N.J. (2015) Study on inclusion of probiotic, prebiotic and its combination in broiler diet and their effect on carcass characteristics and economics of commercial broilers. Vet. World, 8(2): 225-231.
https://doi.org/10.14202/vetworld.2015.225-231
PMid:27047078 PMCid:PMC4774709