Open Access
Research
(Published
online: 08-02-2017)
5.
Evaluation of tissue-engineered bone
constructs using rabbit fetal osteoblasts on acellular bovine
cancellous bone matrix -
Rashmi, Rekha Pathak, Amarpal, H. P. Aithal, P. Kinjavdekar, A. M.
Pawde, A. K. Tiwari, P. Sangeetha, P. Tamilmahan, and A. B.
Manzoor
Veterinary World, 10(2): 163-169
doi:
10.14202/vetworld.2017.163-169
Rashmi:
Division of Veterinary Surgery, Indian Veterinary Research
Institute, Izatnagar, Bareilly - 243 122, Uttar Pradesh, India.
Rekha Pathak:
Division of Veterinary Surgery, Indian Veterinary Research
Institute, Izatnagar, Bareilly - 243 122, Uttar Pradesh, India.
Amarpal:
Division of Veterinary Surgery, Indian Veterinary Research
Institute, Izatnagar, Bareilly - 243 122, Uttar Pradesh, India.
H. P. Aithal:
Division of Veterinary Surgery, Indian Veterinary Research
Institute, Izatnagar, Bareilly - 243 122, Uttar Pradesh, India.
P. Kinjavdekar:
Division of Veterinary Surgery, Indian Veterinary Research
Institute, Izatnagar, Bareilly - 243 122, Uttar Pradesh, India.
A. M. Pawde:
Division of Veterinary Surgery, Indian Veterinary Research
Institute, Izatnagar, Bareilly - 243 122, Uttar Pradesh, India.
A. K. Tiwari:
Division of Standardization, Indian Veterinary Research Institute,
Izatnagar, Bareilly - 243 122, Uttar Pradesh, India.
P. Sangeetha:
Division of Veterinary Surgery, Indian Veterinary Research
Institute, Izatnagar, Bareilly - 243 122, Uttar Pradesh, India.
P. Tamilmahan:
Division of Veterinary Surgery, Indian Veterinary Research
Institute, Izatnagar, Bareilly - 243 122, Uttar Pradesh, India.
A. B. Manzoor:
Division of Veterinary Surgery, Indian Veterinary Research
Institute, Izatnagar, Bareilly - 243 122, Uttar Pradesh, India.
Received: 10-11-2016, Accepted: 04-01-2017, Published online:
08-02-2017
Corresponding author:
Rekha Pathak, e-mail: rekhasurgery@rediffmail.com
Citation:
Rashmi, Pathak R, Amarpal, Aithal HP, Kinjavdekar P, Pawde AM,
Tiwari AK, Sangeetha P, Tamilmahan P, Manzoor AB (2017) Evaluation
of tissue-engineered bone constructs using rabbit fetal
osteoblasts on acellular bovine cancellous bone matrix,
Veterinary World, 10(2): 163-169.
Abstract
Aim:
The aim of this study was to generate composite bone graft and
investigate the rabbit fetal osteoblasts adhesion, proliferation
and penetration on acellular matrices of cancellous bone.
Materials and Methods:
Acellular cancellous bone was prepared and developed as in the
previous study with little modification. These matrices were
decellularized by rapid freeze and thaw cycle. To remove the cell
debris, they were then treated with hydrogen peroxide (3%) and
ethanol to remove antigenic cellular and nuclear materials from
the scaffold. Primary osteoblast cells were harvested from 20 to
22 days old rabbit fetal long and calvarial bone. These cells were
cultured and characterized using a specific marker. The third
passaged fetal osteoblast cells were then seeded on the scaffold
and incubated for 14 days. The growth pattern of the cells was
observed. Scanning electron microscope and hematoxylin and eosin
staining were used to investigate cells proliferation.
Results:
The cells were found to be growing well on the surface of the
scaffold and were also present in good numbers with the matrix
filopodial extensions upto inside of the core of the tissue.
Conclusion:
Thus, a viable composite scaffold of bone could be developed which
has a great potential in the field of bone tissue engineering.
Keywords:
composite grafts, osteoblasts, tissue engineering.
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