Open Access
Research
(Published
online: 30-01-2017)
20.
Associations between single nucleotide
polymorphisms in multiple candidate genes and body weight in
rabbits -
Karim El-Sabrout and Sarah A. Aggag
Veterinary World, 10(1): 136-139
doi:
10.14202/vetworld.2017.136-139
Karim El-Sabrout:
Department of Poultry Production, Faculty of Agriculture,
Aflaton St., El-Shatby, P.O. Box 21545, University of
Alexandria, Alexandria, Egypt.
Sarah A. Aggag:
Department of Genetics, Faculty of Agriculture, Aflaton St., El-Shatby,
P.O. Box 21545, University of Alexandria, Alexandria, Egypt.
Received: 01-10-2016, Accepted: 29-12-2016, Published online:
30-01-2017
Corresponding author:
Karim El-Sabrout, e-mail: kareem.badr@alexu.edu.eg
Citation:
El-Sabrout K, Aggag SA (2017) Associations between single
nucleotide polymorphisms in multiple candidate genes and body
weight in rabbits,
Veterinary World,
10(1): 136-139.
Abstract
Aim:
In this study, we examined parts of six growth genes (growth
hormone [GH], melanocortin 4 receptor [MC4R], growth hormone
receptor [GHR], phosphorglycerate mutase [PGAM], myostatin [MSTN],
and fibroblast growth factor [FGF]) as specific primers for two
rabbit lines (V-line, Alexandria) using nucleotide sequence
analysis, to investigate association between detecting single
nucleotide polymorphism (SNP) of these genes and body weight (BW)
at market.
Materials and Methods:
Each line kits were grouped into high and low weight rabbits to
identify DNA markers useful for association studies with high BW.
DNA from blood samples of each group was extracted to amplify the
six growth genes. SNP technique was used to study the associate
polymorphism in the six growth genes and marketing BW (at 63 days)
in the two rabbit lines. The purified polymerase chain reaction
products were sequenced in those had the highest and lowest BW in
each line.
Results:
Alignment of sequence data from each group revealed the following
SNPs: At nucleotide 23 (A-C) and nucleotide 35 (T-G) in MC4R gene
(sense mutation) of Alexandria and V-line high BW. Furthermore, we
detected the following SNPs variation between the two lines: A SNP
(T-C) at nucleotide 27 was identified by MC4R gene (sense
mutation) and another one (A-C) at nucleotide 14 was identified by
GHR gene (nonsense mutation) of Alexandria line. The results of
individual BW at market (63 days) indicated that Alexandria
rabbits had significantly higher BW compared with V-line rabbits.
MC4R polymorphism showed significant association with high BW in
rabbits.
Conclusion:
The results of polymorphism demonstrate the possibility to detect
an association between BW in rabbits and the efficiency of the
used primers to predict through the genetic specificity using the
SNP of MC4R.
Keywords:
associated genes, body weight, prediction, rabbit, single
nucleotide polymorphism.
References
1. El-Sabrout, K. and Aggag, S.A. (2015) Use of inter simple
sequence repeats and protein markers in assessing genetic
diversity and relationships among four rabbit genotypes. World
Rabbit Sci., 23: 283-288.
https://doi.org/10.4995/wrs.2015.3912 |
|
|
2. Helal, M.A., Mahboub, H.D., Hemeda, S.A., Ballal, S.S. and
Heikal, H.S. (2014) Polymorphism of bone morphogenetic protein
receptor-IB (BMPR-IB) gene with litter size and kids growth of
some goat breeds in Egypt. Alex. J. Vet. Sci., 41(1): 28-34.
https://doi.org/10.5455/ajvs.155470 |
|
|
3. Li, H., Deeb, N., Zhou, H., Mitchell, A.D., Ashwell, C.M.
and Lamont, S.J. (2003) Chicken quantitative trait loci for
growth and body composition associated with transforming
growth factor-β genes. Poult. Sci., 82: 347-356.
https://doi.org/10.1093/ps/82.3.347
PMid:12705392 |
|
|
|
4. Fontanesi, L., Tazzoli, M., Scotti, E. and Russo, V. (2008)
Analysis of Candidate Genes for Meat Production Traits in
Domestic Rabbit Breeds. 9th World Rabbit Congress, Verona,
Italy, A. p79-84. |
|
|
|
5. Amie-Marini, A.B., Aslinda, K., Mohd-Hifzan, R., Muhd-Faisal,
A.B. and Musaddin, K. (2012) HaeIII-RFLP polymorphism of
growth hormone gene in Savanna and Kalahari goats. Malays. J.
Anim. Sci., 15: 13-19. |
|
|
6. Estany, J., Baselga, M., Blasco, A. and Camacho, J. (1989)
Mixed model methodology for the estimation of genetic response
to selection in litter size of rabbits. Livest. Prod. Sci.,
21: 67-75.
https://doi.org/10.1016/0301-6226(89)90021-3 |
|
|
|
7. El-Raffa, A.M. (2007) Formation of a rabbit synthetic line
and primary analysis of its productive and reproductive
performance. Egypt. J. Poult. Sci., 27: 321-334. |
|
|
8. Wallis, O.C. and Wallis, M. (1995) Cloning and
characterisation of the rabbit growth hormone-encoding gene.
Gene, 163: 253-256.
https://doi.org/10.1016/0378-1119(95)00429-A |
|
|
9. Fontanesi, L., Scotti, E., Cisarova, K., Di Battista, P.,
Dall'Olio, S., Fornasini, D. and Frabetti, A. (2013) A
missense mutation in the rabbit melanocortin 4 receptor (MC4R)
gene is associated with finishing weight in a meat rabbit
line. Anim. Biotechnol., 24(4): 268-277.
https://doi.org/10.1080/10495398.2013.781034
PMid:23947663 |
|
|
|
10. Zhang, W.X., Zhang, G.W., Peng, J. and Lai, S.J. (2012)
The Polymorphism of GHR Associated with the Growth and Carcass
Traits in Three Rabbit Breeds. 10th World Rabbit Congress,
Sharm El-Sheikh, Egypt. p75-78. |
|
|
11. Wu, Z.L., Chen, S.Y., Jia, X.B. and Lai, S.J. (2015)
Association of a synonymous mutation of the PGAM2 gene and
growth traits in rabbits. Czech J. Anim. Sci., 60(3): 139-144.
https://doi.org/10.17221/8079-CJAS |
|
|
|
12. Mulsant, P., de Rochambeau, H. and Thébault, R.G. (2004) A
note on linkage between the angora and FGF-5 genes in rabbits.
World Rabbit Sci., 12: 1-6. |
|
|
|
13. GelAnalyzer. (2010) Gel Analyzer software Version 3.1 for
Windows. Available from: http://www.gelanalyzer.com. [Last
accessed on 2016 May 13] |
|
|
14. Zhou, G.L., Jin, H.G., Liu, C., Guo, S.L., Zhu, Q. and Wu,
Y.H. (2005) Association of genetic polymorphism in GH gene
with milk production traits in Beijing Holstein cows. J.
Biosci., 30(5): 595-598.
https://doi.org/10.1007/BF02703558 |
|
|
|
15. SPSS, Statistical Package for the Social Sciences. (2011)
SPSS User's Guide: Statistics, Version 20.0 for Windows.
Institute, Inc., Cary, NC, USA. |
|
|
16. Herrington, J. and Carter-Su, C. (2001) Signaling pathways
activated by the growth hormone receptor. Trends Endocrinol.
Metab., 12: 252-257.
https://doi.org/10.1016/S1043-2760(01)00423-4 |
|
|
17. Sternstein, I., Reissmann, M., Maj, D., Bieniek, J. and
Brockmann, G. (2014) A new single nucleotide polymorphism in
the rabbit (Oryctolagus cuniculus) myostatin (MSTN) gene is
associated with carcass composition traits. Anim. Genet., 45:
596-599.
https://doi.org/10.1111/age.12165
PMid:24796976 |
|
|
|
18. Othman, O., Nowier, A., El-Kader, H.A., Abd El-Aziem, S.
and Youssef, Y. (2015) Genetic characterization of fibroblast
growth factor-5 gene in rabbits. Glob. Vet., 14(4): 453-458. |
|
