Open Access
Research
(Published
online: 12-01-2017)
7.
Incidence and virulence
characteristics of Aeromonas spp. in fish -
Ashraf M. Abd-El-Malek
Veterinary World, 10(1): 34-37
doi:
10.14202/vetworld.2017.34-37
Ashraf M.
Abd-El-Malek:
Department of Food Hygiene (Meat Hygiene), Faculty of Veterinary
Medicine, Assiut University, 71515 Assiut, Egypt.
Received: 20-09-2016, Accepted: 14-12-2016, Published online:
12-01-2017
Corresponding author:
Ashraf M. Abd-El-Malek, e-mail:
ashraf_702001@yahoo.com
Citation:
Abd-El-Malek AM (2017) Incidence and virulence characteristics of
Aeromonas spp. in fish, Veterinary World, 10(1):
34-37.
Abstract
Aim:
This study was
conducted to evaluate the presence of Aeromonas spp. in raw
and ready-to-eat (RTE) fish commonly consumed in Assiut city,
Egypt, and to determine virulence factors due to they play a key
role in their pathogenicity.
Materials and
Methods:
A total of 125
samples of raw and RTE fish samples were taken from different fish
markets and fish restaurants in Assiut Governorate and screened
for the presence of Aeromonas spp. by enrichment on tryptic
soy broth then incubated at 30°C for 24 h. Plating unto the
sterile Petri dishes containing Aeromonas agar base to
which Aeromonas selective supplement was added. The plates
were incubated at 37°C for 24 h. Presumptive Aeromonas
colonies were biochemically confirmed and analyzed for
pathogenicity by hemolysin production, protease, and lipase
detection.
Results:
The results
indicated that raw fish were contaminated with Aeromonas
spp. (40% in wild and 36% in cultured Nile tilapia). Regarding
RTE, Aeromonas spp. could be isolated with the percentage
of 16%, 28% and 20% in fried Bolti, grilled Bolti and fried Bayad,
respectively. Out of 35 isolates obtained, 22 were categorized as
Aeromonas hydrophila, 12 were classified as Aeromonas
sobria and Aeromonas caviae were found in only one
isolate. The virulence factors of Aeromonas spp. were
detected and the results showed that all isolates produced of
hemolysin (91.4%), protease (77.1%), and lipase enzyme (17.1%).
Conclusion:
This
study indicates that the presence of A. hydrophila
with virulence potential in fresh and RTE fish may be a major
threat to public health.
Keywords:
Aeromonas hydrophila,
fish, hemolysin, lipase, protease enzyme, public health.
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