Veterinary World

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Research (Published online: 03-03-2017)

1. Effect of food processing on degradation of hexachlorocyclohexane and its isomers in milk - Sujatha Singh and Krishnaiah Nelapati

Veterinary World, 10(3): 270-275



   doi: 10.14202/vetworld.2017.270-275


Sujatha Singh: Department of Veterinary Public Health and Epidemiology, College of Veterinary Science, P. V. Narsimha Rao Telangana Veterinary University, Rajendranagar, Hyderabad - 500 030, Telangana, India.

Krishnaiah Nelapati: Department of Veterinary Public Health and Epidemiology, College of Veterinary Science, P. V. Narsimha Rao Telangana Veterinary University, Rajendranagar, Hyderabad - 500 030, Telangana, India.


Received: 02-09-2016, Accepted: 24-01-2017, Published online: 03-03-2017


Corresponding author: Sujatha Singh, e-mail:

Citation: Singh S, Nelapati K (2017) Effect of food processing on degradation of hexachlorocyclohexane and its isomers in milk, Veterinary World, 10(3): 270-275.

Aim: To study the effect of different food processing techniques on the degradation of organochlorine compounds (α, β, ɣ and δ hexachlorocyclohexane isomers (HCH)) residues in both natural and fortified samples of milk.

Materials and Methods: Raw milk samples are collected from the local areas of Hyderabad, India. Naturally and fortified milk samples (HCH) were subjected to various food processing techniques, pasteurization (63ºC for ½ h), sterilization (121ºC for 15 min) and boiling for 5 min and analyzed by gas chromatography with electron capture detector using quick, easy, cheap, effective, rugged and safe method for multiresidue analysis of pesticides in milk with slight modification.

Results: The final mean residual concentration of pesticide in milk after heat processing and percentage of degradation were calculated with respective treatments.

Conclusion: Heat treatments are highly effective on reduction of mean residual concentration of HCH in milk. In which Sterilization and boiling proved to be more effective in degradation of HCH isomers.

Keywords: gas chromatography-electron capture detector, hexachlorocyclohexane isomers, pesticide residues, quick; easy; cheap; effective; rugged and safe method.

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