Polymerase chain reaction detection of genes responsible for multiple antibiotic resistance Staphylococcus aureus isolated from food of animal origin in Egypt

Vet World Vol.10 October-2017 Article-8

Research Article

Veterinary World, 10(10): 1205-1211

https://doi.org/10.14202/vetworld.2017.1205-1211

Fawzy R. El Seedy¹, A. A. Samy², Hala S. H. Salam¹, Eman A. Khairy², and Aya A. Koraney²

1. Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt.
2. Department of Microbiology and Immunology, National Research Center, Cairo, Egypt.

Background and Aim: The aim of our study was polymerase chain reaction (PCR) detection of the genes responsible for the multiple antibiotic resistance S. aureus isolated from food of animal origin in Egypt.

Materials and Methods: A total of 125 samples were randomly collected from milk, meat, and their products from Giza and Beni-Suef Governorates markets. The S. aureus isolates were subjected to antimicrobial sensitivity tests using four antibacterial disks (Oxoid), and then the polymerase chain reaction (PCR) was performed for detection of antibiotic resistance genes.

Results: Out of 125 samples, 19 S. aureus isolates were detected. All detected isolates were multiple drug resistance (MDR). The penicillin-, erythromycin-, kanamycin-, and tetracycline-resistant isolates were examined by PCR for resistance genes blaZ, (msrA, ermB, and ermC), aac(6')aph (2''), and tetK. The isolates harbored these resistance genes with percentage of 100% (100%, 0%, and 100%), 62.5%, and 100%, respectively.

Conclusion: Contaminated foods of animal origin may represent a source of MDR S. aureus that can be a major threat to public health. Keywords: food of animal origin, multiple antibiotic resistance, polymerase chain reaction, resistance genes, Staphylococcus aureus.

Keywords: food of animal origin, multiple antibiotic resistance, polymerase chain reaction, resistance genes, Staphylococcus aureus.

How to cite this article: El Seedy FR, Samy AA, Salam HSH, Khairy EA, Koraney AA (2017) Polymerase chain reaction detection of genes responsible for multiple antibiotic resistance Staphylococcus aureus isolated from food of animal origin in Egypt, Veterinary World, 10(10): 1205-1211.

Received: 21-04-2017 Accepted: 11-09-2017 Published online: 09-10-2017

Corresponding author: A. A. Samy E-mail: ayman_samy@hotmail.com

DOI: 10.14202/vetworld.2017.1205-1211

Copyright: El Seedy, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.