Open Access
Research (Published online: 11-10-2017)
9. Protective efficacy of combined trivalent inactivated ISA 71 oil adjuvant vaccine against avian influenza virus subtypes (H9N2 and H5N1) and Newcastle disease virus
Zeinab Mohamed Ali, Mervat Abd El Monaem Hassan, Hussein Ali Hussein, Basem Mohamed Ahmed and Ahmed Abd El-Ghany El Sanousi
Veterinary World, 10(10): 1212-1220

Zeinab Mohamed Ali: Department of Poultry Vaccines, Production Unit Veterinary Serum and Vaccine Research Institute, Abbasia 11759, Egypt.
Mervat Abd El Monaem Hassan: Department of Poultry Vaccines, Production Unit Veterinary Serum and Vaccine Research Institute, Abbasia 11759, Egypt.
Hussein Ali Hussein: Department of Virology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt.
Basem Mohamed Ahmed: Department of Virology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt.
Ahmed Abd El-Ghany El Sanousi: Department of Virology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt.

doi: 10.14202/vetworld.2017.1212-1220

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Article history: Received: 16-06-2017, Accepted: 15-09-2017, Published online: 11-10-2017

Corresponding author: Zeinab Mohamed Ali


Citation: Ali ZM, Hassan MAEM, Hussein HA, Ahmed BM, El Sanousi AAE (2017) Protective efficacy of combined trivalent inactivated ISA 71 oil adjuvant vaccine against avian influenza virus subtypes (H9N2 and H5N1) and Newcastle disease virus, Veterinary World, 10(10): 1212-1220.

Aim: The objective of the present study was to prepare a trivalent inactivated vaccine of Newcastle disease virus (NDV), H5N1, and H9N2 viruses.

Materials and Methods: Three monovalent and a trivalent vaccines were prepared by emulsifying inactivated NDV (LaSota strain), reassortant H5N1, and H9N2 viruses with Montanide ISA 71 oil adjuvant. Parameters used for evaluation of the efficacy of the prepared vaccines in specific pathogen-free chickens were cellular immunity assays (blastogenesis, interferon gamma, interleukin 1 [IL1], and IL6), humoral immunity by hemagglutination inhibition, protection percentage, and shedding.

Results: A single immunization with trivalent vaccine-enhanced cell-mediated immunity as well as humoral immune response with 90% protection against challenges with highly pathogenic avian influenza (HPAI) H5N1 and low pathogenic (LP) avian influenza H9N2 viruses with 100% protection after challenge with NDV.

Conclusion: Development and evaluation of the trivalent vaccine in the study reported the success in preparation of a potent and efficacious trivalent vaccine which is a promising approach for controlling HPAI H5N1, LP H9N2, and ND viral infections.

Keywords: avian influenza, immunization, Newcastle disease virus, shedding, trivalent vaccine.


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