Vet World Vol.10 October-2017 Article-9
Research Article
Veterinary World, 10(10): 1212-1220
https://doi.org/10.14202/vetworld.2017.1212-1220
Protective efficacy of combined trivalent inactivated ISA 71 oil adjuvant vaccine against avian influenza virus subtypes (H9N2 and H5N1) and Newcastle disease virus
2. Department of Virology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt.
Background and Aim: The objective of the present study was to prepare a trivalent inactivated vaccine of Newcastle disease virus (NDV), H5N1, and H9N2 viruses.
Materials and Methods: Three monovalent and a trivalent vaccines were prepared by emulsifying inactivated NDV (LaSota strain), reassortant H5N1, and H9N2 viruses with Montanide ISA 71 oil adjuvant. Parameters used for evaluation of the efficacy of the prepared vaccines in specific pathogen-free chickens were cellular immunity assays (blastogenesis, interferon gamma, interleukin 1 [IL1], and IL6), humoral immunity by hemagglutination inhibition, protection percentage, and shedding.
Results: A single immunization with trivalent vaccine-enhanced cell-mediated immunity as well as humoral immune response with 90% protection against challenges with highly pathogenic avian influenza (HPAI) H5N1 and low pathogenic (LP) avian influenza H9N2 viruses with 100% protection after challenge with NDV.
Conclusion: Development and evaluation of the trivalent vaccine in the study reported the success in preparation of a potent and efficacious trivalent vaccine which is a promising approach for controlling HPAI H5N1, LP H9N2, and ND viral infections. Keywords: avian influenza, immunization, Newcastle disease virus, shedding, trivalent vaccine.
Keywords: avian influenza, immunization, Newcastle disease virus, shedding, trivalent vaccine.
How to cite this article: Ali ZM, Hassan MAEM, Hussein HA, Ahmed BM, El Sanousi AAE (2017) Protective efficacy of combined trivalent inactivated ISA 71 oil adjuvant vaccine against avian influenza virus subtypes (H9N2 and H5N1) and Newcastle disease virus, Veterinary World, 10(10): 1212-1220.
Received: 16-06-2017 Accepted: 15-09-2017 Published online: 11-10-2017
Corresponding author: Zeinab Mohamed Ali E-mail: zizimohamed11@gmail.com
DOI: 10.14202/vetworld.2017.1212-1220
Copyright: Ali, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.