Vet World Vol.12 June-2019 Article-19
Research Article
Veterinary World, 12(6): 849-854
https://doi.org/10.14202/vetworld.2019.849-854
Diabetes sepsis on Wistar rat strain (Rattus norvegicus) induced by streptozotocin and bacteria Staphylococcus aureus
2. Laboratory of Pharmacology, Faculty of Veterinary Medicine, Brawijaya University, Indonesia.
3. Department of Microbiology and Public Health , College of Veterinary Medicine, Tarlac Agricultural University, Camiling, Tarlac, Philippines.
4. Laboratory of Biochemical, Faculty of Veterinary Medicine, Brawijaya University, Indonesia.
Background and Aim: Sepsis is characterized by loss of control of the inflammatory response, which can be triggered by various microorganisms and toxic secretions. The mortality rate increases due to impaired endothelial function caused dysfunctional organ systems. Diabetes is closely related to sepsis. The study aimed to determine the method of using animal models of sepsis diabetes through a combination of streptozotocin (STZ) and Staphylococcus aureus infection based on biological marker parameters.
Materials and Methods: A total of 30 male Wistar rats of 2.5-3 months old weighing approximately 150-250 g body weight (BW) divided into six treatment groups with five replications per group were used in the study. Treatment A was negative control (healthy rats) and Treatment B was the positive control (with diabetes) where rats were given STZ dose at 45 mg/kg BW on day 8 intraperitoneally (IP). The blood glucose was measured on day 10, Treatment C was a positive control (bacteria), rats inoculated with S. aureus with a concentration of 108 CFU/mL on day 8 given IP and observed sepsis conditions on day 10th. Treatment group (D, E, and F): Rats given STZ dose at 45 mg/kg BW on day 8th by IP and measured blood glucose on day 10th, then inoculated with S. aureus with different concentrations of 105 CFU/mL, 106 CFU/mL, and 107 CFU/mL on the 10th day, respectively, and were later observed the condition of sepsis on day 12th. Data on diabetes bacteremia were quantitative used blood glucose levels, the bacterial count, and C-reactive protein (CRP) and were analyzed using the one-way analysis of variance test with a confidence level of 95%. Physical examination (temperature and respiration) is qualitative.
Results: Physical examination showed that all treatments had a normal temperature, an increased pulse in Groups D, E, and F and a decrease in respiratory rate in the treatment of E and F, the bacteria found in the vital organs in all groups, and CRP levels were not significantly different at all.
Conclusion: Animal model of diabetes sepsis can be observed through a combination of pancreas damage, and respiration, the bacteria in the vital organs. Keywords: animal model, diabetes, inflammation, sepsis.
Keywords: animal model, diabetes, inflammation, sepsis.
How to cite this article: Qosimah D, Aryani DE, Beltran MAG, Aulanni'am A (2019) Diabetes sepsis on Wistar rat strain (Rattus norvegicus) induced by streptozotocin and bacteria Staphylococcus aureus, Veterinary World, 12(6): 849-854.
Received: 14-01-2019 Accepted: 18-04-2019 Published online: 19-06-2019
Corresponding author: Dahliatul Qosimah E-mail: dahlia_qosimah@ub.ac.id
DOI: 10.14202/vetworld.2019.849-854
Copyright: Qosimah, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.