Open Access
Research (Published online: 13-09-2019)
5. Bactericidal and virucidal efficacies of food additive grade calcium hydroxide under various concentrations, organic material conditions, exposure duration, and its stability
Sakchai Ruenphet, Kornkamon Paditporn, Darsaniya Punyadarsaniya, Tippawan Jantafong and Kazuaki Takehara
Veterinary World, 12(9): 1383-1389

Sakchai Ruenphet: Department of Immunology and Virology, Faculty of Veterinary Medicine, Mahanakorn University of Technology, Bangkok, Thailand.
Kornkamon Paditporn: Department of Immunology and Virology, Faculty of Veterinary Medicine, Mahanakorn University of Technology, Bangkok, Thailand.
Darsaniya Punyadarsaniya: Department of Immunology and Virology, Faculty of Veterinary Medicine, Mahanakorn University of Technology, Bangkok, Thailand.
Tippawan Jantafong: Department of Immunology and Virology, Faculty of Veterinary Medicine, Mahanakorn University of Technology, Bangkok, Thailand.
Kazuaki Takehara: Laboratory of Animal Health, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, Japan.

doi: 10.14202/vetworld.2019.1383-1389

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Article history: Received: 21-05-2019, Accepted: 29-07-2019, Published online: 13-09-2019

Corresponding author: Sakchai Ruenphet

E-mail: rsakchai@hotmail.com

Citation: Ruenphet S, Paditporn K, Punyadarsaniya D, Jantafong T, Takehara K (2019) Bactericidal and virucidal efficacies of food additive grade calcium hydroxide under various concentrations, organic material conditions, exposure duration, and its stability, Veterinary World, 12(9): 1383-1389.
Abstract

Aim: This study aimed to evaluate the bactericidal and virucidal activity of food additive grade calcium hydroxide (FdCa(OH)2) under various concentrations, organic material conditions, and exposure duration including its stability.

Materials and Methods: The FdCa(OH)2 powder as well as the 0.17% and 3% solutions were evaluated for bacteria and virus inactivating efficacies against Salmonella infantis (SI), Escherichia coli, Newcastle disease virus (NDV), and avian influenza virus (AIV), in the absence or presence of organic materials. In addition, the stability of FdCa(OH)2, was also examined using wet-dry conditions and under sunlight.

Results: The FdCa(OH)2 powder could inactivate both NDV and AIV in the absence and presence of organic materials within a 3 min exposure period. The bactericidal efficacy using solution form revealed that 0.17% and 3% of FdCa(OH)2 could inactivate SI in the absence and presence of organic materials within 3 min of exposure. However, 3% of FdCa(OH)2 inactivated E. coli both with and without organic materials within 3min, while 0.17% required 5 min to be efficacious. The virucidal efficacy also showed that 0.17% FdCa(OH)2 could inactivate NDV in the absence and presence of organic materials within 10 min and 30 min, respectively. However, AIV inactivation was achieved within 30 sec under all conditions. In addition, under wet and dry conditions, FdCa(OH)2 powder demonstrated high efficacy when re-suspended at least 16 times for NDV and 7 times for AIV. Simultaneously, the FdCa(OH)2 powder retained its efficacy under the sunlight during up to 4 months for NDV and at least 6 months for AIV.

Conclusion: The present study indicates that FdCa(OH)2 powder and solutions could inactivate SI, E. coli, NDV, and AIV while retaining good stability under challenging environmental conditions. Finally, the FdCa(OH)2 is safe for consumers because it is of food additive grade and can be useful as an alternative disinfectant, especially for biosecurity enhancement on and around poultry farms.

Keywords: bactericidal, biosecurity, food additive grade calcium hydroxide, virucidal.