Open Access
Research (Published online: 07-10-2020)
8. The distribution pattern and growth factor level in platelet-rich fibrin incorporated skin-derived mesenchymal stem cells: An in vitro study
Igo Syaiful Ihsan, Deya Karsari, Nora Ertanti, Aristika Dinaryanti, Alexander Patera Nugraha, Purwati Purwati, Sri Agus Sudjarwo and Fedik Abdul Rantam
Veterinary World, 13(10): 2097-2103

Igo Syaiful Ihsan: Master Student of Vaccinology and Immunotherapeutica, Veterinary Medicine Faculty, Airlangga University, Surabaya, Indonesia; Stem Cell Research and Development Center, Airlangga University, Surabaya, Indonesia.
Deya Karsari: Stem Cell Research and Development Center, Airlangga University, Surabaya, Indonesia.
Nora Ertanti: Stem Cell Research and Development Center, Airlangga University, Surabaya, Indonesia.
Aristika Dinaryanti: Stem Cell Research and Development Center, Airlangga University, Surabaya, Indonesia.
Alexander Patera Nugraha: Doctoral Student of Medical Science, Faculty of Medicine, Airlangga University, Surabaya, Indonesia.
Purwati Purwati: Stem Cell Research and Development Center, Airlangga University, Surabaya, Indonesia; Department of Health, Vocational Faculty, Airlangga University, Surabaya, Indonesia.
Sri Agus Sudjarwo: Department of Pharmacology, Veterinary Medicine Faculty, Airlangga University, Surabaya, Indonesia.
Fedik Abdul Rantam: Stem Cell Research and Development Center, Airlangga University, Surabaya, Indonesia; Department of Microbiology, Virology Laboratory, Veterinary Medicine Faculty, Airlangga University, Surabaya, Indonesia.

doi: www.doi.org/10.14202/vetworld.2020.2097-2103

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Article history: Received: 15-05-2020, Accepted: 31-08-2020, Published online: 07-10-2020

Corresponding author: Fedik Abdul Rantam

E-mail: fedik-a-r@fkh.unair.ac.id

Citation: Ihsan IS, Karsari D, Ertanti N, Dinaryanti A, Nugraha AP, Purwati P, Sudjarwo SA, Rantam FA (2020) The distribution pattern and growth factor level in platelet-rich fibrin incorporated skin-derived mesenchymal stem cells: An in vitro study, Veterinary World, 13(10): 2097-2103.
Abstract

Background and Aim: A skin wound in an animal must be cared for to prevent further health issues. Platelet-rich fibrin (PRF) and skin-derived mesenchymal stem cells (SMSCs) have been reported to have potential in increasing the rate of wound healing. This study aimed to analyze the distribution patterns and levels of platelet-derived growth factor (PDGF), insulin-like growth factor (IGF), vascular endothelial growth factor (VEGF), and transforming growth factor-β (TGF-β) in PRF incorporated with SMSCs.

Materials and Methods: This study employed a true experiment (in vitro) design with post-test only performed in the control group alone. PRF and SMSCs were extracted from the blood and skin of 16 rabbits. SMSCs were characterized using immunocytochemistry to examine clusters of differentiation for 45, 73, 90, and 105. PRF was incorporated into the SMSCs and then divided into four groups (N=32/n=8): Group A (PRF only), Group B (PRF+SMSCs, incubated for 1 day), Group C (PRF+SMSCs, incubated for 3 days), and Group D (PRF+SMSCs, incubated for 5 days). Scanning electron microscopy was used to examine the distribution pattern of SMSCs between groups. The supernatant serum (Group A) and supernatant medium culture (Group D) were collected for the measurement of PDGF, IGF, VEGF, and TGF-β using an enzyme-linked immunosorbent assay sandwich kit. An unpaired t-test was conducted to analyze the differences between Groups A and D (p<0.01).

Results: Group D had the most morphologically visible SMSCs attached to the PRF, with elongated and pseudopodia cells. There was a significant difference between the levels of growth factor in Groups A and D (p=0.0001; p<0.01).

Conclusion: SMSCs were able to adhere to and distribute evenly on the surface of PRF after 5 days of incubation. The PRF incorporated SMSCs contained high levels of PDGF, IGF, VEGF, and TGF- β, which may prove to have potential in enhancing wound healing.

Keywords: growth factor, platelet-rich fibrin, rabbit, skin mesenchymal stem cells.