Open Access
Research (Published online: 22-09-2020)
27. Isolation and molecular characterization of fowl aviadenovirus associated with inclusion body hepatitis from poultry in Banten and West Java, Indonesia
Otto Sahat Martua Silaen, Sri Murtini, Joko Pamungkas and Christian Marco Hadi Nugroho
Veterinary World, 13(9): 1940-1946

Otto Sahat Martua Silaen: Department of Animal Infectious Disease and Veterinary Public Health, Faculty of Veterinary Medicine, IPB University, Bogor, Indonesia; Animal Health Diagnostic Laboratory, PT Medika Satwa Laboratories, Bogor, Indonesia.
Sri Murtini: Department of Animal Infectious Disease and Veterinary Public Health, Faculty of Veterinary Medicine, IPB University, Bogor, Indonesia.
Joko Pamungkas: Department of Animal Infectious Disease and Veterinary Public Health, Faculty of Veterinary Medicine, IPB University, Bogor, Indonesia.
Christian Marco Hadi Nugroho: Animal Health Diagnostic Laboratory, PT Medika Satwa Laboratories, Bogor, Indonesia.

doi: www.doi.org/10.14202/vetworld.2020.1940-1946

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Article history: Received: 06-05-2020, Accepted: 06-08-2020, Published online: 22-09-2020

Corresponding author: Sri Murtini

E-mail: smurtinifs@yahoo.com

Citation: Silaen OSM, Murtini S, Pamungkas J, Nugroho CMH (2020) Isolation and molecular characterization of fowl aviadenovirus associated with inclusion body hepatitis from poultry in Banten and West Java, Indonesia, Veterinary World, 13(9): 1940-1946.
Abstract

Background and Aim: Fowl avidenoviruses (FAdVs) are generally considered ubiquitous, but certain serotypes and strains are known to be associated with primary diseases, such as inclusion body hepatitis (IBH). Since 2018, the outbreak of IBH has been reported in part provinces of Indonesia. This study aimed to isolate and molecularly characterize the FAdV from Banten and West Java Provinces of Indonesia and described the phylogenetic relationship with the FAdV that has been characterized in other countries.

Materials and Methods: A total of 25 FAdV archive samples have been collected from January to August 2019 from clinical cases of FAdV infection in Banten and West Java Provinces, Indonesia. Collected samples were inoculated in 10-day-old specific-pathogenic-free chicken embryonated eggs. Hexon gene of FAdV was detected using polymerase chain reaction (PCR) with a primer set from previous study. To gain a better understanding of the FAdV genetic properties and construct the phylogeny tree, the PCR products were sequenced and subjected to a BLAST search and inferred using the neighbor-joining method by bootstrap test 1000×.

Results: FAdV-D and FAdV-E are present in Banten, Indonesia. The phylogenetic analysis of 850 nucleotides that encode 289 amino acid of the partial hexon gene shows that the isolates Broiler/MSL/Ciputat-149/18, Broiler/MSL/Lebak-151/18, and Broiler/MSL/Ciputat-29/19 have 100% homology with FAdV-E TR/BVKE/R/D-1 from Turkey, whereas the isolates Layer/MSL/Ciputat-20/19 and Broiler/MSL/Ciputat-30/19 have 100% homology with FAdV-D strain 685 from Canada.

Conclusion: The present study provides updates of the circulating FAdV in commercial poultry flocks in Banten and West Java Provinces, Indonesia. Since the FAdV vaccine was unavailable in Indonesia, this result might be used as guidance to select a proper FAdV vaccine strain. Our result indicates that at least two FAdV species were circulating among poultry in Banten and West Java Provinces, Indonesia; they are FAdV-D and FAdV-E.

Keywords: fowl aviadenovirus, hexon gene, Indonesia, molecular characterization, poultry.