Detection of aerobic bacterial pathogens associated with early embryonic death in pregnant New Zealand female Rabbits in Egypt

Research (Published online: 24-04-2021)

25. Detection of aerobic bacterial pathogens associated with early embryonic death in pregnant New Zealand female Rabbits in Egypt

Heba Roshdy, Azhar G. Shalaby, Ahmed Abd Elhalem Mohamed and Heba Badr

Veterinary World, 14(4): 986-995

Heba Roshdy: Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Agricultural Research Center, Nadi El-Seid Street, Dokki P.O. Box246, Giza 12618, Egypt.

Azhar G. Shalaby: Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Agricultural Research Center, Nadi El-Seid Street, Dokki P.O. Box246, Giza 12618, Egypt.

Ahmed Abd Elhalem Mohamed: Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Agricultural Research Center, Nadi El-Seid Street, Dokki P.O. Box246, Giza 12618, Egypt.

Heba Badr: Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Agricultural Research Center, Nadi El-Seid Street, Dokki P.O. Box246, Giza 12618, Egypt.

doi: www.doi.org/10.14202/vetworld.2021.986-995

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Article history: Received: 23-11-2020, Accepted: 08-03-2021, Published online: 24-04-2021

Corresponding author: Heba Badr

E-mail: drheba_badr@yahoo.com

Citation: Roshdy H, Shalaby AG, Mohamed AAE, Badr H (2021) Detection of aerobic bacterial pathogens associated with early embryonic death in pregnant New Zealand female Rabbits in Egypt, Veterinary World, 14(4): 986-995.

Abstract

Background and Aim: Rabbits are a highly sensitive species and susceptible to various bacterial pathogens that may be causative agents for early embryonic death. This study aimed to explore the administration of different bacterial agents in does suffering from early embryonic death. Furthermore, identification of genes associated with virulence was performed to identify the phenotypic and genotypic antimicrobial resistance patterns that may increase the virulence of pathogens and lead to early embryonic death.

Materials and Methods: We isolated and identified bacterial agents in 106 samples from live and dead female rabbits that had undergone early embryonic death, including liver and intestine tissue, aborted fetuses, discharges, and vaginal swabs. Conventional polymerase chain reaction (PCR) was conducted to confirm the identity of the isolated bacterial strains and their virulence. Moreover, antibiotic resistance was studied phenotypically and genotypically.

Results: We isolated Escherichia coli, Salmonella, Staphylococcus aureus, Pasteurella multocida, and Listeria monocytogenes. PCR confirmed typical identification except in P. multocida, which was confirmed as Gallibacterium spp. in some cases. The final percentage of detection was 34%, 30.2%, 16.9%, 13.2%, and 11.3%, respectively. Virulence properties were investigated using different designated genes. All Salmonella strains harbored invA, stn, avrA, and ompf genes, while the sopE gene was identified in 31.25%. E. coli strains harboring the iss gene lacked the shiga toxin (stx1) gene. L. monocytogenes and S. aureus strains harbored the hemolysin gene (66.7% and 33.4%, respectively). Multidrug resistance was detected phenotypically and genotypically in most strains. Each bacterial pathogen had a different antibiotic resistance profile.

Conclusion: Multiple bacterial species may contribute to early embryonic death in does. Furthermore, the combined infection could be the main cause of early embryonic death. Thus, monitoring programs should bear this in mind and focus on the early detection of these bacterial agents in female rabbits to avoid embryonic death.

Keywords: early embryonic death, Escherichia coli, Listeria, Rabbits, Salmonella, Staphylococcus aureus.