Open Access
Research (Published online: 08-03-2021)
5. Accuracy and precision guidelines for optimal breeding time in bitches using in-house progesterone measurement compared with chemiluminescent microparticle immunoassay
Nutnaree Kunanusont, Darsaniya Punyadarsaniya and Sakchai Ruenphet
Veterinary World, 14(3): 585-588

Nutnaree Kunanusont: Clinic for Horse, Faculty of Veterinary Medicine, Mahanakorn University of Technology, Bangkok, Thailand.
Darsaniya Punyadarsaniya: Department of Immunology and Virology, Veterinary Medicine Faculty, Mahanakorn University of Technology, Bangkok, Thailand.
Sakchai Ruenphet: Department of Immunology and Virology, Veterinary Medicine Faculty, Mahanakorn University of Technology, Bangkok, Thailand.

doi: www.doi.org/10.14202/vetworld.2021.585-588

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Article history: Received: 08-10-2020, Accepted: 27-01-2021, Published online: 08-03-2021

Corresponding author: Sakchai Ruenphet

E-mail: rsakchai@hotmail.com

Citation: Kunanusont N, Punyadarsaniya D, Ruenphet S (2021) Accuracy and precision guidelines for optimal breeding time in bitches using in-house progesterone measurement compared with chemiluminescent microparticle immunoassay, Veterinary World, 14(3): 585-588.
Abstract

Background and Aim: The concentration of serum progesterone is commonly used to determine the optimal mating time in bitches, and to diagnose reproductive-related abnormalities. This study aims to compare the serum progesterone results obtained by rapid fluorescence immunochromatography assay (RFICA) with those obtained by chemiluminescent microparticle immunoassay (CMIA) from the same serum samples to develop a standard guideline for optimal breeding time.

Materials and Methods: Serum progesterone levels were measured in 124 bitches using RFICA and CMIA. Simple linear regression and correlation analyses were performed to analyze the data. The percentage difference between the maximum and minimum progesterone values in the same serum sample in the same assay was compared using Wilcoxon's rank-sum test.

Results: The present study showed a strong linear dependence of the results obtained by RFICA on those obtained by CMIA as R2=0.8976, with regression coefficient of 0.9474 and p<0.05, including the regression model was CMIA = (0.9483 × RFICA) - 0.761. Moreover, five critical measurement times during estrous in bitches showed statistically significant differences (p<0.05), except at the fertilizable period, which showed a non-significant difference (p>0.05).

Conclusion: This study demonstrated that it is presumably acceptable to use the RFICA and CMIA methods interchangeably for quality progesterone measurements in serum samples from bitches. However, when considering the use of the RFICA method, it is advisable to carefully interpret the results and follow the interpretation guidelines. Finally, RFICA in the present study provides a reliable and convenient option for veterinarian practitioners to measure canine progesterone levels in-house.

Keywords: bitches, chemiluminescent microparticle immunoassay, optimal breed timing, progesterone, rapid fluorescence immunochromatography assay.