Open Access
Research (Published online: 07-10-2021)
6. Actinomycetes of secondary metabolite producers from mangrove sediments, Central Java, Indonesia
Wilis Ari Setyati, Delianis Pringgenies, Nirwani Soenardjo and Rini Pramesti
Veterinary World, 14(10): 2620-2624

Wilis Ari Setyati: Department of Marine Science, Faculty of Fisheries and Marine Science, Diponegoro University, Semarang, Central Java, 50275, Indonesia.
Delianis Pringgenies: Department of Marine Science, Faculty of Fisheries and Marine Science, Diponegoro University, Semarang, Central Java, 50275, Indonesia.
Nirwani Soenardjo: Department of Marine Science, Faculty of Fisheries and Marine Science, Diponegoro University, Semarang, Central Java, 50275, Indonesia.
Rini Pramesti: Department of Marine Science, Faculty of Fisheries and Marine Science, Diponegoro University, Semarang, Central Java, 50275, Indonesia.

doi: www.doi.org/10.14202/vetworld.2021.2620-2624

Share this article on [Facebook] [LinkedIn]

Article history: Received: 06-06-2021, Accepted: 01-09-2021, Published online: 07-10-2021

Corresponding author: Wilis Ari Setyati

E-mail: wilisarisetyati@yahoo.co.id

Citation: Setyati WA, Pringgenies D, Soenardjo N, Pramesti R (2021) Actinomycetes of secondary metabolite producers from mangrove sediments, Central Java, Indonesia, Veterinary World, 14(10): 2620-2624.
Abstract

Background and Aim: Actinomycetes are a group of Gram-positive bacteria with a fungus-like morphology. Their natural habitat encompasses terrestrial and water areas, including mangrove ecosystems. This study aimed to assess the PKS and NRPS genes as the producers of secondary metabolites and to determine the target bacterial species using molecular DNA tests.

Materials and Methods: In this study, we isolated bacteria from sediment samples from mangrove forests located on Karimunjawa Islands and in Semarang city, purified bacteria, screened for antibacterial activity, extracted bacterial DNA, amplified the NRPS gene, detected and amplified the PKS-I and PKS-II genes, amplified and sequenced the 16S rRNA, processed molecular data, and simulated a map of secondary metabolite producing genes.

Results: Samples from the Karimunjawa Islands yielded 19 bacterial isolates, whereas samples from Semarang yielded 11 bacterial isolates after culture in different media. Further experiments identified three active isolates, which were termed PN.SB.6.2, S.SK.6.3, and S.SK.7.1, against pathogenic species of Escherichia coli, Staphylococcus aureus, and Listeria monocytogenes. Isolate PN.SB.6.2 was determined to possess three biosynthetic gene clusters (BGCs), whereas the remaining two isolates, S.SK.6.3 and S.SK.7.1, only possessed two BGCs, namely, NRPS and PKS II.

Conclusion: Products were estimated to be in the NRPS, thiopeptide, RiPP-like, siderophore, betalactone, terpene, Type III PKS, CDPS, and lassopeptide groups. DNA identification of the isolates found three species of actinomycetes with antibacterial potential, namely, Virgibacillus salaries, Bacillus licheniformis, and Priestia flexa.

Keywords: actinomycetes, biosynthetic gene cluster, Karimunjawa Island, NRPS, PKS.