Open Access
Research (Published online: 27-01-2023)
21. Antibiotic resistance of Escherichia coli from the milk of Ettawa crossbred dairy goats in Blitar Regency, East Java, Indonesia
Tweedekharis Marlin Agatha, Prima Ayu Wibawati, Reza Ikhza Izulhaq, Bodhi Agustono, Ragil Angga Prastiya, Dhandy Koesoemo Wardhana, Abzal Abdramanov, Widya Paramita Lokapirnasari, and Mirni Lamid
Veterinary World, 16(1): 168-174

Tweedekharis Marlin Agatha: Department of Veterinary Medicine, School of Health and Life Sciences (SIKIA), Universitas Airlangga, Surabaya 60115, Indonesia.
Prima Ayu Wibawati: Department of Veterinary Medicine, School of Health and Life Sciences (SIKIA), Universitas Airlangga, Surabaya 60115, Indonesia.
Reza Ikhza Izulhaq: Department of Veterinary Medicine, School of Health and Life Sciences (SIKIA), Universitas Airlangga, Surabaya 60115, Indonesia.
Bodhi Agustono: Department of Veterinary Medicine, School of Health and Life Sciences (SIKIA), Universitas Airlangga, Surabaya 60115, Indonesia.
Ragil Angga Prastiya: Department of Veterinary Medicine, School of Health and Life Sciences (SIKIA), Universitas Airlangga, Surabaya 60115, Indonesia.
Dhandy Koesoemo Wardhana: Department of Veterinary Science, Division of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya 60115, Indonesia.
Abzal Abdramanov: Department of Veterinary Sanitary Expertise and Hygiene, Faculty of Veterinary medicine, Kazakh National Agrarian Research University, Almaty, Kazakhstan.
Widya Paramita Lokapirnasari: Department of Veterinary Science, Division of Animal Husbandry, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya 60115, Indonesia.
Mirni Lamid: Department of Veterinary Science, Division of Animal Husbandry, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya 60115, Indonesia.

doi: www.doi.org/10.14202/vetworld.2023.168-174

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Article history: Received: 26-08-2022, Accepted: 02-12-2022, Published online: 27-01-2023

Corresponding author: Prima Ayu Wibawati

E-mail: primaayuwibawati@fkh.unair.ac.id

Citation: Agatha TM, Wibawati PA, Izulhaq RI, Agustono B, Prastiya RA, Wardhana DK, Abdramanov A, Lokapirnasari WP, and Lamid M (2023) Antibiotic resistance of Escherichia coli from the milk of Ettawa crossbred dairy goats in Blitar Regency, East Java, Indonesia, Veterinary World, 16(1): 168–174.
Abstract

Background and Aim: Antimicrobial resistance, especially antibiotic resistance, is one of the most severe public health challenges. Antibiotic resistance occurs when bacteria avoid and fight the mechanism of action of antibiotic drugs. This study aimed to determine the resistance of Escherichia coli from the milk of Ettawa crossbreed dairy goat at Blitar Regency, East Java, Indonesia, with the antibiotics streptomycin, sulfonamides, and trimethoprim.

Materials and Methods: A total of 34 milk samples of Ettawa crossbreed dairy goats were used in this study. The initial stages of this research included tests of the physical properties, isolation, and identification of E. coli. Then, the E. coli isolates were tested for antibiotic resistance using the Kirby–Bauer method.

Results: The results showed that all samples were positive for E. coli. The physical properties of milk, namely, color, odor, flavor, and consistency, were normal. The results of the alcohol test showed normal acidity, and the specific gravity of goat milk met the criteria, with an average specific gravity of 1.0295 g/mL. The results of the antibiotic resistance test showed that 4 (12%) samples were resistant to streptomycin, 5 (15%) to sulfonamide, and 3% to trimethoprim.

Conclusion: The prevalence of E. coli from Ettawa crossbreed dairy goats in Blitar Regency, East Java, Indonesia, was 100%. Furthermore, this E. coli isolate exhibited resistance to antibiotics streptomycin, sulfonamides, and trimethoprim. The use of antibiotics in the dairy goat industry in Indonesia should be controlled to prevent the spread of resistant E. coli from animals to humans through the food chain and prevent the emergence of multidrug-resistant E. coli.

Keywords: antibiotic drugs, Escherichia coli, Indonesia, public health, specific gravity.