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Research
2.
Cytopathic effect of PPR vaccine virus
strains in vero cells -
Raveendra Hegde, Amitha R. Gomes, S. M. Byregowda, A. K.
Santhosh and C. Renukaprasad
Vet World. 2009; 2(3): 93-94
Abstract
The present
study describes the cytopathic effect of two different Peste des
petits ruminants (PPR) vaccine virus strains presently being
used in the country, in vero cells. The cytopathic effect (CPE)
was visible from 4th
day post infection in Sungri vaccine virus strain where as
Arasur vaccine virus strain showed CPE, 36-48 hr post infection.
With both vaccine virus strains the CPE in vero cells showed
initial cell rounding, aggregation and syncytial development.
The generalized CPE was noticed by 6th
day in Sungri and by 96 hrs post infection in Arasur strain.
However complete detachment of the cell monolayer was observed
in Arasur strain by 120 hr, post infection. Infected coverslip
cultures stained with H & E and May & Grunwald’s Giemsa showed
cell vaculation, cytoplasmic extension and syncytia comprising
of five to six nuclei. Acidophilic intracytoplasmic and
intranuclear inclusion bodies were also observed. Titers, HA
activity and detection by s-ELISA of both the vaccine virus
strains are also compared.
Keywords:
Vaccine, Cytopathic Effect, Virus Strain, Vero Cells, Peste des
Petits.
